The secret lives of fluorescent membrane probes as revealed by molecular dynamics simulations

HAL Filipe, MJ Moreno, LMS Loura - Molecules, 2020 - mdpi.com
Fluorescent probes have been employed for more than half a century to study the structure
and dynamics of model and biological membranes, using spectroscopic and/or microscopic …

Physical properties of the cytoplasm modulate the rates of microtubule polymerization and depolymerization

AT Molines, J Lemière, M Gazzola, IE Steinmark… - Developmental Cell, 2022 - cell.com
The cytoplasm is a crowded, visco-elastic environment whose physical properties change
according to physiological or developmental states. How the physical properties of the …

Exploring the Depth-Dependent Microviscosity inside a Micelle Using Butterfly-Motion-Based Fluorescent Probes

X Chen, S Yuan, M Qiao, X Jin, J Chen… - Journal of the …, 2023 - ACS Publications
The viscosity distribution of micellar interiors from the very center to the outer surface is
dramatically varied, which has been distinguished in theoretical models, yet it remains …

Multichannel fluorescence microscopy: advantages of going beyond a single emission

P Rodríguez-Sevilla, SA Thompson… - Advanced NanoBiomed …, 2022 - Wiley Online Library
Fluorescent microscopy has enabled the study of intracellular processes and revealed the
most intricate details of the subcellular structure. This has benefitted not only the basic …

Directly imaging emergence of phase separation in peroxidized lipid membranes

M Paez-Perez, A Vyšniauskas, I López-Duarte… - Communications …, 2023 - nature.com
Lipid peroxidation is a process which is key in cell signaling and disease, it is exploited in
cancer therapy in the form of photodynamic therapy. The appearance of hydrophilic moieties …

Effect of lipid raft disruptors on cell membrane fluidity studied by fluorescence spectroscopy

Á Horváth, J Erostyák, É Szőke - International Journal of Molecular …, 2022 - mdpi.com
Lipid rafts are specialized microdomains in cell membranes, rich in cholesterol and
sphingolipids, and play an integrative role in several physiological and pathophysiological …

Interaction and antibacterial activity of ciprofloxacin with choline based ionic liquid and CTAB: A comparative spectroscopic study

AR Bhat, M ud din Parray, K Imtiyaz, MMA Rizvi… - … Acta Part A: Molecular …, 2024 - Elsevier
In this study, the complexation of potential chemo-therapeutic antibacterial drug,
ciprofloxacin (CIP) with varying concentrations of surface active compounds (SACs) ie,(N-(2 …

Azobenzene photoisomerization probes cell membrane viscosity

A Magni, G Bondelli, GM Paternò, S Sardar… - Physical Chemistry …, 2022 - pubs.rsc.org
The viscosity of cell membranes is a crucial parameter that affects the diffusion of small
molecules both across and within the lipid membrane and that is related to several diseases …

NAD (P) H binding configurations revealed by time-resolved fluorescence and two-photon absorption

TS Blacker, MR Duchen, AJ Bain - Biophysical Journal, 2023 - cell.com
NADH and NADPH play key roles in the regulation of metabolism. Their endogenous
fluorescence is sensitive to enzyme binding, allowing changes in cellular metabolic state to …

A time-correlated single photon counting SPAD array camera with a bespoke data-processing algorithm for lightsheet fluorescence lifetime imaging (FLIM) and FLIM …

J Nedbal, F Mattioli Della Rocca, IT Ivanova, A Allan… - Scientific Reports, 2024 - nature.com
A wide-field microscope with epi-fluorescence and selective plane illumination was
combined with a single-photon avalanche diode (SPAD) array camera to enable live-cell …