Recent advances in interrogating RNA folding dynamics have shown the classical model of
RNA folding to be incomplete. Here, we pose three prominent questions for the field that are …

Ribonucleoprotein (RNP) assembly typically begins during transcription when folding of the
newly synthesized RNA is coupled with the recruitment of RNA-binding proteins (RBPs) …

In prokaryotes, translation can occur on mRNA that is being transcribed in a process called
coupling. How the ribosome affects the RNA polymerase (RNAP) during coupling is not well …

The series of RNA folding events that occur during transcription can critically influence
cellular RNA function. Here, we present reconstructing RNA dynamics from data (R2D2), a …

RNA begins to fold as it is transcribed by an RNA polymerase. Consequently, RNA folding is
constrained by the direction and rate of transcription. Understanding how RNA folds into …

Owing to their unique abilities to manipulate, label, and image individual molecules in vitro
and in cellulo, single-molecule techniques provide previously unattainable access to …

The advent of single-molecule force spectroscopy represents the introduction of forces,
torques, and displacements as controlled variables in biochemistry. These methods afford …

Biomolecular interactions are at the base of all physical processes within living organisms;
the study of these interactions has led to the development of a plethora of different methods …

Many heat shock genes in bacteria are regulated through a class of temperature-sensitive
stem-loop (SL) RNAs called RNA thermometers (RNATs). One of the most widely studied …

Mechanistic details of the signal recognition particle (SRP)-mediated insertion of membrane
proteins have been described from decades of in vitro biochemical studies. However, the …