Metabolite–enzyme coevolution: from single enzymes to metabolic pathways and networks

L Noda-Garcia, W Liebermeister… - Annual Review of …, 2018 - annualreviews.org
How individual enzymes evolved is relatively well understood. However, individual enzymes
rarely confer a physiological advantage on their own. Judging by its current state, the …

Computational approaches to predict protein functional families and functional sites

C Rauer, N Sen, VP Waman, M Abbasian… - Current Opinion in …, 2021 - Elsevier
Understanding the mechanisms of protein function is indispensable for many biological
applications, such as protein engineering and drug design. However, experimental …

Revealing unexplored sequence-function space using sequence similarity networks

JN Copp, E Akiva, PC Babbitt, N Tokuriki - Biochemistry, 2018 - ACS Publications
The rapidly expanding number of protein sequences found in public databases can improve
our understanding of how protein functions evolve. However, our current knowledge of …

Multi-omics data integration analysis identifies the spliceosome as a key regulator of DNA double-strand break repair

D Sherill-Rofe, O Raban, S Findlay, D Rahat… - NAR …, 2022 - academic.oup.com
DNA repair by homologous recombination (HR) is critical for the maintenance of genome
stability. Germline and somatic mutations in HR genes have been associated with an …

Exploring the sequence, function, and evolutionary space of protein superfamilies using sequence similarity networks and phylogenetic reconstructions

JN Copp, DW Anderson, E Akiva, PC Babbitt… - Methods in …, 2019 - Elsevier
Integrative computational methods can facilitate the discovery of new protein functions and
enzymatic reactions by enabling the observation and investigation of complex sequence …

General strategies for using amino acid sequence data to guide biochemical investigation of protein function

EN Kennedy, CA Foster, SA Barr… - Biochemical Society …, 2022 - portlandpress.com
The rapid increase of '-omics' data warrants the reconsideration of experimental strategies to
investigate general protein function. Studying individual members of a protein family is likely …

New computational approaches to understanding molecular protein function

JS Fetrow, PC Babbitt - PLoS Computational Biology, 2018 - journals.plos.org
Function is like beauty—its definition lies in the eye of the beholder or, in this case, the
researcher. At the broadest level, we define organismal function—the function that the …

An atlas of peroxiredoxins created using an active site profile-based approach to functionally relevant clustering of proteins

AF Harper, JB Leuthaeuser, PC Babbitt… - PLoS Computational …, 2017 - journals.plos.org
Peroxiredoxins (Prxs or Prdxs) are a large protein superfamily of antioxidant enzymes that
rapidly detoxify damaging peroxides and/or affect signal transduction and, thus, have roles …

PASV: Automatic protein partitioning and validation using conserved residues

RM Moore, AO Harrison, DJ Nasko, J Chopyk… - bioRxiv, 2021 - biorxiv.org
Background Increasingly, researchers use protein-coding genes from targeted PCR
amplification or direct metagenomic sequencing in community and population ecology …

Isofunctional clustering and conformational analysis of the arsenate reductase superfamily reveals nine distinct clusters

MR Rosen, JB Leuthaeuser, CA Parish, JS Fetrow - Biochemistry, 2020 - ACS Publications
Arsenate reductase (ArsC) is a superfamily of enzymes that reduce arsenate. Due to active
site similarities, some ArsC can function as low-molecular weight protein tyrosine …