Improving cell recovery: freezing and thawing optimization of induced pluripotent stem cells

M Uhrig, F Ezquer, M Ezquer - Cells, 2022 - mdpi.com
Achieving good cell recovery after cryopreservation is an essential process when working
with induced pluripotent stem cells (iPSC). Optimized freezing and thawing methods are …

Dense optical flow software to quantify cellular contractility

S Scalzo, MQL Afonso, NJ da Fonseca, ICG Jesus… - Cell Reports …, 2021 - cell.com
Cell membrane deformation is an important feature that occurs during many physiological
processes, and its study has been put to good use to investigate cardiomyocyte function …

[HTML][HTML] Non-permissive SARS-CoV-2 infection in human neurospheres

C da SG Pedrosa, L Goto-Silva, JR Temerozo… - Stem Cell Research, 2021 - Elsevier
Abstract Coronavirus disease 2019 (COVID-19) was initially described as a viral infection of
the respiratory tract. It is now known, however, that several other organs are affected …

[HTML][HTML] Microscopy-based cellular contractility assay for adult, neonatal, and hiPSC cardiomyocytes

S Scalzo, CATF Mendonça, C Kushmerick, U Agero… - STAR protocols, 2022 - Elsevier
This protocol provides instructions to acquire high-quality cellular contractility data from
adult, neonatal, and human induced pluripotent stem cell-derived cardiomyocytes …

[HTML][HTML] A Novel Recombinant Vitronectin Variant Supports the Expansion and Differentiation of Pluripotent Stem Cells in Defined Animal-Free Workflows

X Lu, E Perr, T Naqvi, D Galitz, M Andersen… - Cells, 2024 - pmc.ncbi.nlm.nih.gov
An essential aspect of harnessing the potential of pluripotent stem cells (PSCs) and their
derivatives for regenerative medicine is the development of animal-free and chemically …

Laminin–dynamic bonds enable multifunctionality in a biological 2D network

T Bhuvanesh, Y Nie, R Machatschek… - Advanced Functional …, 2023 - Wiley Online Library
A layer of laminins, assembled on a thin sheet of collagen type IV (Col‐IV) forms the
backbone of the basal lamina, which controls biological processes such as embryogenesis …

In situ maturated early-stage human-induced pluripotent stem cell-derived cardiomyocytes improve cardiac function by enhancing segmental contraction in infarcted …

D Biagi, ET Fantozzi, JC Campos-Oliveira… - Journal of Personalized …, 2021 - mdpi.com
The scant ability of cardiomyocytes to proliferate makes heart regeneration one of the
biggest challenges of science. Current therapies do not contemplate heart re …

[PDF][PDF] Role of modern technologies in tissue engineering

Q Ali, S Malik, A Malik, MN Hafeez… - Archives of …, 2020 - brieflands.com
: Nanotechnology is a rapidly developing field with numerous applications spread in
different fields, especially medicine. It plays a role in tissue engineering, tissue regeneration …

[HTML][HTML] Modeling of endothelial cell dysfunction using human induced pluripotent stem cells derived from patients with end-stage renal disease

KW Kim, YJ Shin, BM Kim, S Cui, EJ Ko… - Kidney Research and …, 2021 - ncbi.nlm.nih.gov
Background Endothelial cell (EC) dysfunction is a frequent feature in patients with end-stage
renal disease (ESRD). The aim of this study was to generate human induced pluripotent …

Action potential variability in human pluripotent stem cell-derived cardiomyocytes obtained from healthy donors

AB Carvalho, KCS Coutinho, RAQ Barbosa… - Frontiers in …, 2022 - frontiersin.org
Human pluripotent stem cells (PSC) have been used for disease modelling, after
differentiation into the desired cell type. Electrophysiologic properties of cardiomyocytes …