Improving cell recovery: freezing and thawing optimization of induced pluripotent stem cells
Achieving good cell recovery after cryopreservation is an essential process when working
with induced pluripotent stem cells (iPSC). Optimized freezing and thawing methods are …
with induced pluripotent stem cells (iPSC). Optimized freezing and thawing methods are …
Dense optical flow software to quantify cellular contractility
Cell membrane deformation is an important feature that occurs during many physiological
processes, and its study has been put to good use to investigate cardiomyocyte function …
processes, and its study has been put to good use to investigate cardiomyocyte function …
[HTML][HTML] Non-permissive SARS-CoV-2 infection in human neurospheres
C da SG Pedrosa, L Goto-Silva, JR Temerozo… - Stem Cell Research, 2021 - Elsevier
Abstract Coronavirus disease 2019 (COVID-19) was initially described as a viral infection of
the respiratory tract. It is now known, however, that several other organs are affected …
the respiratory tract. It is now known, however, that several other organs are affected …
[HTML][HTML] Microscopy-based cellular contractility assay for adult, neonatal, and hiPSC cardiomyocytes
This protocol provides instructions to acquire high-quality cellular contractility data from
adult, neonatal, and human induced pluripotent stem cell-derived cardiomyocytes …
adult, neonatal, and human induced pluripotent stem cell-derived cardiomyocytes …
[HTML][HTML] A Novel Recombinant Vitronectin Variant Supports the Expansion and Differentiation of Pluripotent Stem Cells in Defined Animal-Free Workflows
X Lu, E Perr, T Naqvi, D Galitz, M Andersen… - Cells, 2024 - pmc.ncbi.nlm.nih.gov
An essential aspect of harnessing the potential of pluripotent stem cells (PSCs) and their
derivatives for regenerative medicine is the development of animal-free and chemically …
derivatives for regenerative medicine is the development of animal-free and chemically …
Laminin–dynamic bonds enable multifunctionality in a biological 2D network
T Bhuvanesh, Y Nie, R Machatschek… - Advanced Functional …, 2023 - Wiley Online Library
A layer of laminins, assembled on a thin sheet of collagen type IV (Col‐IV) forms the
backbone of the basal lamina, which controls biological processes such as embryogenesis …
backbone of the basal lamina, which controls biological processes such as embryogenesis …
In situ maturated early-stage human-induced pluripotent stem cell-derived cardiomyocytes improve cardiac function by enhancing segmental contraction in infarcted …
D Biagi, ET Fantozzi, JC Campos-Oliveira… - Journal of Personalized …, 2021 - mdpi.com
The scant ability of cardiomyocytes to proliferate makes heart regeneration one of the
biggest challenges of science. Current therapies do not contemplate heart re …
biggest challenges of science. Current therapies do not contemplate heart re …
[PDF][PDF] Role of modern technologies in tissue engineering
: Nanotechnology is a rapidly developing field with numerous applications spread in
different fields, especially medicine. It plays a role in tissue engineering, tissue regeneration …
different fields, especially medicine. It plays a role in tissue engineering, tissue regeneration …
[HTML][HTML] Modeling of endothelial cell dysfunction using human induced pluripotent stem cells derived from patients with end-stage renal disease
KW Kim, YJ Shin, BM Kim, S Cui, EJ Ko… - Kidney Research and …, 2021 - ncbi.nlm.nih.gov
Background Endothelial cell (EC) dysfunction is a frequent feature in patients with end-stage
renal disease (ESRD). The aim of this study was to generate human induced pluripotent …
renal disease (ESRD). The aim of this study was to generate human induced pluripotent …
Action potential variability in human pluripotent stem cell-derived cardiomyocytes obtained from healthy donors
AB Carvalho, KCS Coutinho, RAQ Barbosa… - Frontiers in …, 2022 - frontiersin.org
Human pluripotent stem cells (PSC) have been used for disease modelling, after
differentiation into the desired cell type. Electrophysiologic properties of cardiomyocytes …
differentiation into the desired cell type. Electrophysiologic properties of cardiomyocytes …