For years, it has been held that cathepsin D (CD) is involved in rather non-specific protein
degradation in a strongly acidic milieu of lysosomes. Studies with CD knock-out mice …

The lysosomal aspartic protease cathepsin D (cath-D) is over-expressed and hyper-secreted
by epithelial breast cancer cells. This protease is an independent marker of poor prognosis …

In LAMP-2–deficient mice autophagic vacuoles accumulate in many tissues, including liver,
pancreas, muscle, and heart. Here we extend the phenotype analysis using cultured …

Mannose 6-phosphate-dependent transport of soluble lysosomal enzymes is a crucial step
in the biogenesis of lysosomes. Newly synthesized lysosomal enzymes are carried from …

To investigate the question of whether lytic granules share a common biogenesis with
lysosomes, cloned cytolytic T cell lines were derived from a patient with I-cell disease. The …

Publisher Summary This chapter describes the isolation and characterization of the
mannose receptor protein, the cell biology of its involvement in endocytosis and …

Recombinant latent human procathepsin B produced in yeast was purified to near
homogeneity. The purified recombinant proenzyme is activated in vitro under acidic …

A biomarker is an analyte indicating the presence of a biological process linked to the
clinical manifestations and outcome of a particular disease. In the case of lysosomal storage …

Lysosomal hydrolases have long been known to be responsible for the degradation of
different substrates in the cell. These acid hydrolases are synthesized in the rough …

The current mechanism proposed for the processing and activation of the 52 kDa lysosomal
aspartic protease cathepsin D (cath-D) is a combination of partial auto-activation generating …