Metagenomics-enabled microbial surveillance

KKK Ko, KR Chng, N Nagarajan - Nature Microbiology, 2022 - nature.com
Lessons learnt from the COVID-19 pandemic include increased awareness of the potential
for zoonoses and emerging infectious diseases that can adversely affect human health …

Genomics in the long-read sequencing era

EL van Dijk, D Naquin, K Gorrichon, Y Jaszczyszyn… - Trends in Genetics, 2023 - cell.com
Long-read sequencing (LRS) technologies have provided extremely powerful tools to
explore genomes. While in the early years these methods suffered technical limitations, they …

Oxford Nanopore R10. 4 long-read sequencing enables the generation of near-finished bacterial genomes from pure cultures and metagenomes without short-read or …

M Sereika, RH Kirkegaard, SM Karst, TY Michaelsen… - Nature …, 2022 - nature.com
Abstract Long-read Oxford Nanopore sequencing has democratized microbial genome
sequencing and enables the recovery of highly contiguous microbial genomes from isolates …

[HTML][HTML] Expansion of the global RNA virome reveals diverse clades of bacteriophages

U Neri, YI Wolf, S Roux, AP Camargo, B Lee… - Cell, 2022 - cell.com
High-throughput RNA sequencing offers broad opportunities to explore the Earth RNA
virome. Mining 5,150 diverse metatranscriptomes uncovered> 2.5 million RNA virus contigs …

High-quality metagenome assembly from long accurate reads with metaMDBG

G Benoit, S Raguideau, R James, AM Phillippy… - Nature …, 2024 - nature.com
We introduce metaMDBG, a metagenomics assembler for PacBio HiFi reads. MetaMDBG
combines a de Bruijn graph assembly in a minimizer space with an iterative assembly over …

Metagenome assembly of high-fidelity long reads with hifiasm-meta

X Feng, H Cheng, D Portik, H Li - Nature methods, 2022 - nature.com
De novo assembly of metagenome samples is a common approach to the study of microbial
communities. Current metagenome assemblers developed for short sequence reads or …

Nanopore long-read-only metagenomics enables complete and high-quality genome reconstruction from mock and complex metagenomes

L Liu, Y Yang, Y Deng, T Zhang - Microbiome, 2022 - Springer
Background The accurate and comprehensive analyses of genome-resolved metagenomics
largely depend on the reconstruction of reference-quality (complete and high-quality) …

RefSeq and the prokaryotic genome annotation pipeline in the age of metagenomes

DH Haft, A Badretdin, G Coulouris… - Nucleic Acids …, 2024 - academic.oup.com
Abstract The Reference Sequence (RefSeq) project at the National Center for Biotechnology
Information (NCBI) contains over 315 000 bacterial and archaeal genomes and 236 million …

Capturing the microbial dark matter in desert soils using culturomics-based metagenomics and high-resolution analysis

S Li, WH Lian, JR Han, M Ali, ZL Lin, YH Liu… - npj Biofilms and …, 2023 - nature.com
Deserts occupy one-third of the Earth's terrestrial surface and represent a potentially
significant reservoir of microbial biodiversity, yet the majority of desert microorganisms …

Identification of errors in draft genome assemblies at single-nucleotide resolution for quality assessment and improvement

K Li, P Xu, J Wang, X Yi, Y Jiao - Nature Communications, 2023 - nature.com
Assembly of a high-quality genome is important for downstream comparative and functional
genomic studies. However, most tools for genome assembly assessment only give …