Current progress in expansion microscopy: chemical strategies and applications

G Wen, V Leen, T Rohand, M Sauer… - Chemical …, 2023 - ACS Publications
Expansion microscopy (ExM) is a newly developed super-resolution technique, allowing
visualization of biological targets at nanoscale resolution on conventional fluorescence …

Expansion microscopy: super-resolution imaging with hydrogels

S Truckenbrodt - Analytical Chemistry, 2023 - ACS Publications
Guidelines, Not Rules 14 Chemical Principles of Accessing Biomolecules in Expansion
Microscopy 14 Accessing Proteins 14 Protein Anchoring with Indirect Readout 14 Protein …

CCDC15 localizes to the centriole inner scaffold and controls centriole length and integrity

MD Arslanhan, S Cengiz-Emek, E Odabasi… - Journal of Cell …, 2023 - rupress.org
Centrioles are microtubule-based organelles responsible for forming centrosomes and cilia,
which serve as microtubule-organizing, signaling, and motility centers. Biogenesis and …

Plasmodium ARK2 and EB1 drive unconventional spindle dynamics, during chromosome segregation in sexual transmission stages

M Zeeshan, E Rea, S Abel, K Vukušić, R Markus… - Nature …, 2023 - nature.com
The Aurora family of kinases orchestrates chromosome segregation and cytokinesis during
cell division, with precise spatiotemporal regulation of its catalytic activities by distinct protein …

Expansion Microscopy Reveals Plasmodium falciparum Blood-Stage Parasites Undergo Anaphase with A Chromatin Bridge in the Absence of Mini-Chromosome …

B Liffner, S Absalon - Microorganisms, 2021 - mdpi.com
The malaria parasite Plasmodium falciparum undergoes closed mitosis, which occurs within
an intact nuclear envelope, and differs significantly from its human host. Mitosis is …

Epitope-preserving magnified analysis of proteome (eMAP)

J Park, S Khan, DH Yun, T Ku, KL Villa, JE Lee… - Science …, 2021 - science.org
Synthetic tissue-hydrogel methods have enabled superresolution investigation of biological
systems using diffraction-limited microscopy. However, chemical modification by fixatives …

Motor usage imprints microtubule stability along the shaft

M Andreu-Carbó, S Fernandes, MC Velluz, K Kruse… - Developmental cell, 2022 - cell.com
Tubulin dimers assemble into dynamic microtubules, which are used by molecular motors as
tracks for intracellular transport. Organization and dynamics of the microtubule network are …

[PDF][PDF] An extended DNA-free intranuclear compartment organizes centrosome microtubules in malaria parasites

CS Simon, C Funaya, J Bauer, Y Voβ… - Life science …, 2021 - life-science-alliance.org
Plasmodium falciparum encounters significant population bottlenecks when being
transmitted between humans and mosquitoes. To overcome those, it undergoes several …

A multicellular developmental program in a close animal relative

M Olivetta, C Bhickta, N Chiaruttini, J Burns, O Dudin - Nature, 2024 - nature.com
All animals develop from a single-celled zygote into a complex multicellular organism
through a series of precisely orchestrated processes,. Despite the remarkable conservation …

Human SFI1 and Centrin form a complex critical for centriole architecture and ciliogenesis

MH Laporte, IB Bouhlel, E Bertiaux, CG Morrison… - The EMBO …, 2022 - embopress.org
Over the course of evolution, the centrosome function has been conserved in most
eukaryotes, but its core architecture has evolved differently in some clades, with the …