Microbial biofilms: biosurfactants as antibiofilm agents

IM Banat, MAD De Rienzo, GA Quinn - Applied microbiology and …, 2014 - Springer
Current microbial inhibition strategies based on planktonic bacterial physiology have been
known to have limited efficacy on the growth of biofilm communities. This problem can be …

Application of flow cytometry to industrial microbial bioprocesses

M Díaz, M Herrero, LA García, C Quirós - Biochemical engineering journal, 2010 - Elsevier
Flow cytometry (FC) is a powerful technique for the rapid analysis of single cells in a mixture,
by means of light-scattering and fluorescence measurements. In biotechnology, the power of …

Critical aspects of using bacterial cell viability assays with the fluorophores SYTO9 and propidium iodide

P Stiefel, S Schmidt-Emrich, K Maniura-Weber, Q Ren - BMC microbiology, 2015 - Springer
Background Viability staining with SYTO9 and propidium iodide (PI) is a frequently used tool
in microbiological studies. However, data generated by such routinely used method are …

A new noninvasive model to study the effectiveness of dentin disinfection by using confocal laser scanning microscopy

J Ma, Z Wang, Y Shen, M Haapasalo - Journal of endodontics, 2011 - Elsevier
INTRODUCTION: The present study was designed to develop a standardized model for
quantification of the effectiveness of dentin disinfection by different antibacterial solutions …

Detection of pathological biomarkers in human clinical samples via amplifying genetic switches and logic gates

A Courbet, D Endy, E Renard, F Molina… - Science translational …, 2015 - science.org
Whole-cell biosensors have several advantages for the detection of biological substances
and have proven to be useful analytical tools. However, several hurdles have limited whole …

Evaluating metabolic stress and plasmid stability in plasmid DNA production by Escherichia coli

F Silva, JA Queiroz, FC Domingues - Biotechnology advances, 2012 - Elsevier
In the context of recombinant DNA technology, the development of feasible and high-
yielding plasmid DNA production processes has regained attention as more evidence for its …

Applications of flow cytometry to characterize bacterial physiological responses

V Ambriz-Aviña, JA Contreras-Garduño… - BioMed research …, 2014 - Wiley Online Library
Although reports of flow cytometry (FCM) applied to bacterial analysis are increasing,
studies of FCM related to human cells still vastly outnumber other reports. However, current …

Heightened reactive oxygen species generation in the antimicrobial activity of a three component iodinated chitosan− silver nanoparticle composite

M Banerjee, S Mallick, A Paul, A Chattopadhyay… - Langmuir, 2010 - ACS Publications
Herein we report synergy in antimicrobial activity of a chitosan− silver nanoparticle (CS− Ag
NP) composite in the presence of molecular iodine. Green fluorescent protein (GFP) …

New device for high-throughput viability screening of flow biofilms

MR Benoit, CG Conant, C Ionescu-Zanetti… - Applied and …, 2010 - Am Soc Microbiol
Control of biofilms requires rapid methods to identify compounds effective against them and
to isolate resistance-compromised mutants for identifying genes involved in enhanced …

Fewer bacteria adhere to softer hydrogels

KW Kolewe, SR Peyton… - ACS applied materials & …, 2015 - ACS Publications
Clinically, biofilm-associated infections commonly form on intravascular catheters and other
hydrogel surfaces. The overuse of antibiotics to treat these infections has led to the spread of …