Single‐cell RNA sequencing technologies and applications: A brief overview

D Jovic, X Liang, H Zeng, L Lin, F Xu… - Clinical and …, 2022 - Wiley Online Library
Single‐cell RNA sequencing (scRNA‐seq) technology has become the state‐of‐the‐art
approach for unravelling the heterogeneity and complexity of RNA transcripts within …

Charting plant gene functions in the multi-omics and single-cell era

T Depuydt, B De Rybel, K Vandepoele - Trends in Plant Science, 2023 - cell.com
Despite the increased access to high-quality plant genome sequences, the set of genes with
a known function remains far from complete. With the advent of novel bulk and single-cell …

The single-cell stereo-seq reveals region-specific cell subtypes and transcriptome profiling in Arabidopsis leaves

K Xia, HX Sun, J Li, J Li, Y Zhao, L Chen, C Qin… - Developmental Cell, 2022 - cell.com
Understanding the complex functions of plant leaves requires a thorough characterization of
discrete cell features. Although single-cell gene expression profiling technologies have …

Single-cell RNA sequencing provides a high-resolution roadmap for understanding the multicellular compartmentation of specialized metabolism

S Sun, X Shen, Y Li, Y Li, S Wang, R Li, H Zhang… - Nature plants, 2023 - nature.com
Monoterpenoid indole alkaloids (MIAs) are among the most diverse specialized metabolites
in plants and are of great pharmaceutical importance. We leveraged single-cell …

Single-cell transcriptome atlas and chromatin accessibility landscape reveal differentiation trajectories in the rice root

TQ Zhang, Y Chen, Y Liu, WH Lin, JW Wang - Nature communications, 2021 - nature.com
Root development relies on the establishment of meristematic tissues that give rise to
distinct cell types that differentiate across defined temporal and spatial gradients. Dissection …

Single‐cell RNA‐seq reveals fate determination control of an individual fibre cell initiation in cotton (Gossypium hirsutum)

Y Qin, M Sun, W Li, M Xu, L Shao, Y Liu… - Plant Biotechnology …, 2022 - Wiley Online Library
Cotton fibre is a unicellular seed trichome, and lint fibre initials per seed as a factor
determines fibre yield. However, the mechanisms controlling fibre initiation from ovule …

A rice single cell transcriptomic atlas defines the developmental trajectories of rice floret and inflorescence meristems

J Zong, L Wang, L Zhu, L Bian, B Zhang… - New …, 2022 - Wiley Online Library
Rice inflorescence development determines yield and relies on the activity of axillary
meristems (AMs); however, high‐resolution analysis of its early development is lacking …

Asymmetric gene expression and cell-type-specific regulatory networks in the root of bread wheat revealed by single-cell multiomics analysis

L Zhang, C He, Y Lai, Y Wang, L Kang, A Liu, C Lan… - Genome biology, 2023 - Springer
Background Homoeologs are defined as homologous genes resulting from allopolyploidy.
Bread wheat, Triticum aestivum, is an allohexaploid species with many homoeologs …

Single-cell transcriptomic analysis reveals the developmental trajectory and transcriptional regulatory networks of pigment glands in Gossypium bickii

Y Sun, Y Han, K Sheng, P Yang, Y Cao, H Li, QH Zhu… - Molecular Plant, 2023 - cell.com
Comprehensive utilization of cottonseeds is limited by the presence of pigment glands and
its inclusion gossypol. The ideal cotton has glandless seeds but a glanded plant, a trait …

Integrative omics approaches for biosynthetic pathway discovery in plants

KS Singh, JJJ van der Hooft, SCM van Wees… - Natural Product …, 2022 - pubs.rsc.org
Covering: up to 2022 With the emergence of large amounts of omics data, computational
approaches for the identification of plant natural product biosynthetic pathways and their …