Concerns over climate change have necessitated a rethinking of our transportation
infrastructure. One possible alternative to carbon-polluting fossil fuels is biofuels produced …

Studying cell metabolism serves a plethora of objectives such as the enhancement of
bioprocess performance, and advancement in the understanding of cell biology, of drug …

The yeast Saccharomyces cerevisiae is one of the oldest and most frequently used
microorganisms in biotechnology with successful applications in the production of both bulk …

Pichia pastoris is one of the most widely used host for the production of recombinant
proteins. Expression systems that rely mostly on promoters from genes encoding alcohol …

Metabolic networks are regulated to ensure the dynamic adaptation of biochemical reaction
fluxes to maintain cell homeostasis and optimal metabolic fitness in response to …

The genomic era has revolutionized research on secondary metabolites and bioinformatics
methods have in recent years revived the antibiotic discovery process after decades with …

Mathematical models of the cellular metabolism have become an essential tool for the
optimization of biotechnological processes. They help to obtain a systemic understanding of …

Microbial fermentation processes are expected to play an important role in reducing
dependence on fossil-based raw materials for the production of everyday chemicals. In …

Elementary flux modes (EFMs) emerged as a formal concept to describe metabolic
pathways and have become an established tool for constraint-based modeling and …

Highlights•Reaction directionalities versus enzyme reversibility: role of thermodynamics.•
Assessment of methods for thermodynamics-based analysis of metabolic …