De novo purification scheme and crystallization conditions yield high-resolution structures of chitinase A and its complex with the inhibitor allosamidin

Y Papanikolau, G Tavlas, CE Vorgias… - … Section D: Biological …, 2003 - journals.iucr.org
The purification scheme of chitinase A (ChiA) from S. marcescens has been extensively
revised. The pure enzyme crystallizes readily under new crystallization conditions. The ChiA …

[PDF][PDF] Principles of recombinant protein production, extraction and purification from bacterial strains

E Khaled - 2015 - researchgate.net
The biotechnology field intends to produce recombinant proteins from bacteria, which can
be made in far greater abundance than many native proteins [1]. There are a number of …