DNA transfer in forensic science: a review

RAH Van Oorschot, B Szkuta, GE Meakin… - Forensic Science …, 2019 - Elsevier
Understanding the variables impacting DNA transfer, persistence, prevalence and recovery
(DNA-TPPR) has become increasingly relevant in investigations of criminal activities to …

Recent trends and developments in forensic DNA extraction

KWY Chong, Z Thong, CKC Syn - … Reviews: Forensic Science, 2021 - Wiley Online Library
The extraction of DNA is a fairly standard procedure routinely undertaken in biological
academic and research settings. In a forensic DNA context, DNA extraction is the crucial first …

[HTML][HTML] Single-cell transcriptome sequencing allows genetic separation, characterization and identification of individuals in multi-person biological mixtures

L Kulhankova, D Montiel González, E Bindels… - Communications …, 2023 - nature.com
Identifying individuals from biological mixtures to which they contributed is highly relevant in
crime scene investigation and various biomedical research fields, but despite previous …

A systematic approach to improve downstream single‐cell analysis for the DEPArray™ technology

J Schulte, MA Marciano, E Scheurer… - Journal of Forensic …, 2023 - Wiley Online Library
Most commercially available STR amplification kits have never been fully validated for low
template DNA analysis, highlighting the need for testing different PCR kits and conditions for …

[HTML][HTML] Revisiting single cell analysis in forensic science

DRL Watkins, D Myers, HE Xavier, MA Marciano - Scientific Reports, 2021 - nature.com
Forensic science has yet to take full advantage of single cell analysis. Its greatest benefit is
the ability to alleviate the challenges associated with DNA mixture analysis, which remains a …

Analytical approaches to differential extraction for sexual assault evidence

C Clark, R Turiello, R Cotton, JP Landers - Analytica chimica acta, 2021 - Elsevier
Many forensic laboratories face growing demands for the processing of DNA evidence from
sexual assault investigations. In these cases, evidence collected from the crime scene or …

Single cell genomics applications in forensic science: Current state and future directions

K Huffman, J Ballantyne - Iscience, 2023 - cell.com
Standard methods of mixture analysis involve subjecting a dried crime scene sample to
a'bulk'DNA extraction method such that the resulting isolate compromises a homogenized …

[HTML][HTML] Pushing the boundaries: forensic DNA phenotyping challenged by single-cell sequencing

M Diepenbroek, B Bayer, K Anslinger - Genes, 2021 - mdpi.com
Single-cell sequencing is a fast developing and very promising field; however, it is not
commonly used in forensics. The main motivation behind introducing this technology into …

Y‐STR mixture deconvolution by single‐cell analysis

K Huffman, E Hanson… - Journal of Forensic …, 2023 - Wiley Online Library
Since Y‐STR typing only amplifies male Y chromosomal DNA, it can simplify the
interpretation of some DNA mixtures that contain female DNA. However, if there are multiple …

[HTML][HTML] Optical tweezers as an effective tool for spermatozoa isolation from mixed forensic samples

N Auka, M Valle, BD Cox, PD Wilkerson… - PLoS …, 2019 - journals.plos.org
A single focus optical tweezer is formed when a laser beam is launched through a high
numerical aperture immersion objective. This objective focuses the beam down to a …