Efficient transformation of numerous important crops remains a challenge, due
predominantly to our inability to stimulate growth of transgenic cells capable of producing …

Since the success of Agrobacterium-mediated transformation of rice in the early 1990s,
significant advances in Agrobacterium-mediated transformation of monocotyledonous plant …

Maize ARGOS 8 is a negative regulator of ethylene responses. A previous study has shown
that transgenic plants constitutively overexpressing ARGOS 8 have reduced ethylene …

Targeted DNA double-strand breaks have been shown to significantly increase the
frequency and precision of genome editing. In the past two decades, several double-strand …

While transformation of the major monocot crops is currently possible, the process typically
remains confined to one or two genotypes per species, often with poor agronomics, and …

Targeted mutagenesis, editing of endogenous maize (Zea mays) genes, and site-specific
insertion of a trait gene using clustered regularly interspaced short palindromic repeats …

Genome editing using sequence-specific nucleases (SSNs) is rapidly being developed for
genetic engineering in crop species. The utilization of zinc finger nucleases (ZFNs) …

Class 2 CRISPR systems are exceptionally diverse, nevertheless, all share a single effector
protein that contains a conserved RuvC-like nuclease domain. Interestingly, the size of these …

Background Agroinfiltration is a simple and effective method of delivering transgenes into
plant cells for the rapid production of recombinant proteins and has become the preferred …

Background Somatic embryogenesis is a major process for plant regeneration. However,
cell communication and the gene regulatory network responsible for cell reprogramming …