Single-molecule localization microscopy (SMLM) describes a family of powerful imaging
techniques that dramatically improve spatial resolution over standard, diffraction-limited …

Optical contrasts in microscopy are sensitive to light polarization, whose interaction with
molecular dipoles provides an important lever for probing molecular orientation. Polarization …

Quantitative fluorescence and superresolution microscopy are often limited by insufficient
data quality or artifacts. In this context, it is essential to have biologically relevant control …

Lamella micromachining by focused ion beam milling at cryogenic temperature (cryo-FIB)
has matured into a preparation method widely used for cellular cryo-electron tomography …

Combining the molecular specificity of fluorescent probes with three-dimensional imaging at
nanoscale resolution is critical for investigating the spatial organization and interactions of …

Single-molecule localization microscopy is a powerful tool for visualizing subcellular
structures, interactions and protein functions in biological research. However …

Single molecule localization microscopy offers in principle resolution down to the molecular
level, but in practice this is limited primarily by incomplete fluorescent labeling of the …

Mammalian cells use diverse pathways to prevent deleterious consequences during DNA
replication, yet the mechanism by which cells survey individual replisomes to detect …

Quantitative data analysis is important for any single-molecule localization microscopy
(SMLM) workflow to extract biological insights from the coordinates of the single …

Multifocal plane microscopy allows for capturing images at different focal planes
simultaneously. Using a proprietary prism which splits the emitted light into paths of different …