Proteins adopt different higher-order structures (HOS) to enable their unique biological
functions. Understanding the complexities of protein higher-order structures and dynamics …

Diazirines are three-membered ring systems containing two nitrogen atoms and one carbon
atom1, 2 widely applicable in biology as photoaffinity labeling agents. 3, 4 Diazirines can be …

Target identification is often the rate‐determining step in deciphering the mechanism of
action of biologically active small molecules. Photo‐affinity labeling (PAL) represents a …

Studies on fluorinated inhalation anesthetics, including synthesis, physical chemistry and
pharmacology, have been summarized in this review. Retrospecting the history of inhalation …

Sevoflurane is a commonly used inhaled general anesthetic. Despite this, its mechanism of
action remains largely elusive. Compared to other anesthetics, sevoflurane exhibits distinct …

We identified a fluorophore, 1-aminoanthracene (1-AMA), that is anesthetic, potentiates
GABAergic transmission, and gives an appropriate dissociation constant, K d≈ 0.1 mM, for …

Volatility and low-affinity hamper an ability to define molecular targets of the inhaled
anesthetics. Photolabels have proven to be a useful approach in this regard, although none …

Isothermal titration calorimetry (ITC) is a fast and robust method to determine the energetics
of association reactions in solution. The changes in enthalpy, entropy and heat capacity that …

General anesthetic photolabels are used to reveal molecular targets and molecular binding
sites of anesthetic ligands. After identification, the relevance of anesthetic substrates or …

Photolabeling has allowed considerable progress in the understanding of anesthetic
binding to proteins, of target identity, and of site localization. There are, however, few groups …