During the last ten years, developments in cryo–electron microscopy have transformed our
understanding of eukaryotic ribosome assembly. As a result, the field has advanced from a …

Translation termination is an essential cellular process, which is also of therapeutic interest
for diseases that manifest from premature stop codons. In eukaryotes, translation termination …

Integral membrane proteins (IMPs) play central roles in cellular physiology and represent
the majority of known drug targets. Single-molecule fluorescence and fluorescence …

The CCR4-NOT complex is a major regulator of eukaryotic messenger RNA (mRNA)
stability. Slow decoding during translation promotes association of CCR4-NOT with …

The protein translocon at the endoplasmic reticulum comprises the Sec61 translocation
channel and numerous accessory factors that collectively facilitate the biogenesis of …

While lysine methylation is well-known for regulating gene expression transcriptionally, its
implications in translation have been largely uncharted. Trimethylation at lysine 22 …

General protein folding is mediated by chaperones that utilize ATP hydrolysis to regulate
client binding and release. Zinc-finger protein 1 (Zpr1) is an essential ATP-independent …

Reversible modification of target proteins by ubiquitin and ubiquitin-like proteins (UBLs) is
widely used by eukaryotic cells to control protein fate and cell behaviour. UFM1 is a UBL that …

Ribosomal action is facilitated by the orchestrated work of trans-acting factors and ribosomal
elements, which are subject to regulatory events, often involving phosphorylation. One such …

The functional diversity of RNAs is encoded in their innate conformational heterogeneity.
The combination of single-molecule spectroscopy and computational modeling offers new …