PCR inhibition in qPCR, dPCR and MPS—mechanisms and solutions
DNA analysis has seen an incredible development in terms of instrumentation, assays and
applications over the last years. Massively parallel sequencing (MPS) and digital PCR are …
applications over the last years. Massively parallel sequencing (MPS) and digital PCR are …
[HTML][HTML] qPCR primer design revisited
Primers are arguably the single most critical components of any PCR assay, as their
properties control the exquisite specificity and sensitivity that make this method uniquely …
properties control the exquisite specificity and sensitivity that make this method uniquely …
Real-time PCR detection chemistry
E Navarro, G Serrano-Heras, MJ Castaño, J Solera - Clinica chimica acta, 2015 - Elsevier
Real-time PCR is the method of choice in many laboratories for diagnostic and food
applications. This technology merges the polymerase chain reaction chemistry with the use …
applications. This technology merges the polymerase chain reaction chemistry with the use …
Mechanisms of inactivation by high-voltage atmospheric cold plasma differ for Escherichia coli and Staphylococcus aureus
Atmospheric cold plasma (ACP) is a promising nonthermal technology effective against a
wide range of pathogenic microorganisms. Reactive oxygen species (ROS) play a crucial …
wide range of pathogenic microorganisms. Reactive oxygen species (ROS) play a crucial …
[HTML][HTML] Xenobiotics shape the physiology and gene expression of the active human gut microbiome
The human gut contains trillions of microorganisms that influence our health by metabolizing
xenobiotics, including host-targeted drugs and antibiotics. Recent efforts have characterized …
xenobiotics, including host-targeted drugs and antibiotics. Recent efforts have characterized …
[图书][B] Experimental methods in wastewater treatment
Over the past twenty years, the knowledge and understanding of wastewater treatment has
advanced extensively and moved away from empirically based approaches to a …
advanced extensively and moved away from empirically based approaches to a …
Circulating miRNA analysis for cancer diagnostics and therapy
Successful treatment of cancer depends on early diagnosis and effective monitoring of
patients' response to therapy. Traditional tools based on tumor biopsies lack the sensitivity …
patients' response to therapy. Traditional tools based on tumor biopsies lack the sensitivity …
Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data
JM Ruijter, C Ramakers, WMH Hoogaars… - Nucleic acids …, 2009 - academic.oup.com
Despite the central role of quantitative PCR (qPCR) in the quantification of mRNA
transcripts, most analyses of qPCR data are still delegated to the software that comes with …
transcripts, most analyses of qPCR data are still delegated to the software that comes with …
PrimerBank: a resource of human and mouse PCR primer pairs for gene expression detection and quantification
A Spandidos, X Wang, H Wang… - Nucleic acids research, 2010 - academic.oup.com
ABSTRACT PrimerBank (http://pga. mgh. harvard. edu/primerbank/) is a public resource for
the retrieval of human and mouse primer pairs for gene expression analysis by PCR and …
the retrieval of human and mouse primer pairs for gene expression analysis by PCR and …
The real-time polymerase chain reaction
M Kubista, JM Andrade, M Bengtsson… - Molecular aspects of …, 2006 - Elsevier
The scientific, medical, and diagnostic communities have been presented the most powerful
tool for quantitative nucleic acids analysis: real-time PCR [Bustin, SA, 2004. A–Z of …
tool for quantitative nucleic acids analysis: real-time PCR [Bustin, SA, 2004. A–Z of …