Precise control of gene expression is fundamental to cell function and development.
Although ultimately gene expression relies on DNA-binding transcription factors to guide the …

Comparative genome analyses reveal that organismal complexity scales not with gene
number but with gene regulation. Recent efforts indicate that the human genome likely …

Transcription of highly expressed genes has been shown to occur in stochastic bursts. But
the origin of such ubiquitous phenomenon has not been understood. Here, we present the …

Accurate measurements of promoter activities are crucial for predictably building genetic
systems. Here we report a method to simultaneously count plasmid DNA, RNA transcripts …

Loading of the ring-shaped Mcm2–7 replicative helicase around DNA licenses eukaryotic
origins of replication. During loading, Cdc6, Cdt1, and the origin-recognition complex (ORC) …

Bacterial enhancer binding proteins (bEBPs) are transcriptional activators that assemble as
hexameric rings in their active forms and utilize ATP hydrolysis to remodel the conformation …

Transcription initiation is a highly regulated step of gene expression. Here, we discuss the
series of large conformational changes set in motion by initial specific binding of bacterial …

The integration of synthetic and cell-free biology has made tremendous strides towards
creating artificial cellular nanosystems using concepts from solution-based chemistry, where …

Genomic DNA is a crowded track where motor proteins frequently collide. It remains
underexplored whether these collisions carry physiological function. In this work, we …

Molecular motors are diverse enzymes that transduce chemical energy into mechanical
work and, in doing so, perform critical cellular functions such as DNA replication and …