Shotgun metagenomics, from sampling to analysis

C Quince, AW Walker, JT Simpson, NJ Loman… - Nature …, 2017 - nature.com
Diverse microbial communities of bacteria, archaea, viruses and single-celled eukaryotes
have crucial roles in the environment and in human health. However, microbes are …

DIAMOND+ MEGAN: fast and easy taxonomic and functional analysis of short and long microbiome sequences

C Bağcı, S Patz, DH Huson - Current protocols, 2021 - Wiley Online Library
One main approach to computational analysis of microbiome sequences is to first align
against a reference database of annotated protein sequences (NCBI‐nr) and then perform …

Succession and persistence of microbial communities and antimicrobial resistance genes associated with International Space Station environmental surfaces

NK Singh, JM Wood, F Karouia, K Venkateswaran - Microbiome, 2018 - Springer
Abstract Background The International Space Station (ISS) is an ideal test bed for studying
the effects of microbial persistence and succession on a closed system during long space …

Single cell genomics reveals plastid-lacking Picozoa are close relatives of red algae

ME Schön, VV Zlatogursky, RP Singh, C Poirier… - Nature …, 2021 - nature.com
The endosymbiotic origin of plastids from cyanobacteria gave eukaryotes photosynthetic
capabilities and launched the diversification of countless forms of algae. These primary …

Translational informatics for human microbiota: data resources, models and applications

K Shen, AU Din, B Sinha, Y Zhou… - Briefings in …, 2023 - academic.oup.com
With the rapid development of human intestinal microbiology and diverse microbiome-
related studies and investigations, a large amount of data have been generated and …

ezTree: an automated pipeline for identifying phylogenetic marker genes and inferring evolutionary relationships among uncultivated prokaryotic draft genomes

YW Wu - BMC genomics, 2018 - Springer
Background Inferring phylogenetic trees for newly recovered genomes from metagenomic
samples is very useful in determining the identities of uncultivated microorganisms. Even …

Introduction to the analysis of environmental sequences: metagenomics with MEGAN

C Bağcı, S Beier, A Górska, DH Huson - Evolutionary genomics: statistical …, 2019 - Springer
Metagenomics has become a part of the standard toolkit for scientists interested in studying
microbes in the environment. Compared to 16S rDNA sequencing, which allows coarse …

A multi-source domain annotation pipeline for quantitative metagenomic and metatranscriptomic functional profiling

A Ugarte, R Vicedomini, J Bernardes, A Carbone - Microbiome, 2018 - Springer
Background Biochemical and regulatory pathways have until recently been thought and
modelled within one cell type, one organism and one species. This vision is being …

Identifying accurate metagenome and amplicon software via a meta-analysis of sequence to taxonomy benchmarking studies

PP Gardner, RJ Watson, XC Morgan, JL Draper… - PeerJ, 2019 - peerj.com
Metagenomic and meta-barcode DNA sequencing has rapidly become a widely-used
technique for investigating a range of questions, particularly related to health and …

Performance of multiple metagenomics pipelines in understanding microbial diversity of a low-biomass spacecraft assembly facility

JM Wood, NK Singh, L Guan, A Seuylemezian… - Frontiers in …, 2021 - frontiersin.org
NASA planetary protection (PP) requires an assessment of the biological contamination of
the potential microbial burden on spacecraft destined to explore planetary bodies that may …