Zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs)
comprise a powerful class of tools that are redefining the boundaries of biological research …

Programmable nucleases—including zinc-finger nucleases (ZFNs), transcription activator-
like effector nucleases (TALENs) and RNA-guided engineered nucleases (RGENs) derived …

Nucleases that cleave unique genomic sequences in living cells can be used for targeted
gene editing and mutagenesis. Here we develop a strategy for generating such reagents …

Reverse genetics in model organisms such as Drosophila melanogaster, Arabidopsis
thaliana, zebrafish and rats, efficient genome engineering in human embryonic stem and …

Transcription activator-like effector nucleases (TALENs) are programmable nucleases that
join FokI endonuclease with the modular DNA-binding domain of TALEs. Although zinc …

Clinical-grade T cells are genetically modified ex vivo to express a chimeric antigen receptor
(CAR) to redirect specificity to a tumor associated antigen (TAA) thereby conferring antitumor …

Saturation mutagenesis,—coupled to an appropriate biological assay—represents a
fundamental means of achieving a high-resolution understanding of regulatory and protein …

Efficient strategies for precise genome editing in human-induced pluripotent cells (hiPSCs)
will enable sophisticated genome engineering for research and clinical purposes. The …

The transfer of high-avidity T cell receptor (TCR) genes isolated from rare tumor-specific
lymphocytes into polyclonal T cells is an attractive cancer immunotherapy strategy …

Present adoptive immunotherapy strategies are based on the re-targeting of autologous T-
cells to recognize tumor antigens. As T-cell properties may vary significantly between …