Metabolic glycoengineering–exploring glycosylation with bioorthogonal chemistry
M Kufleitner, LM Haiber, V Wittmann - Chemical Society Reviews, 2023 - pubs.rsc.org
Glycans are involved in numerous biological recognition events. Being secondary gene
products, their labeling by genetic methods–comparable to GFP labeling of proteins–is not …
products, their labeling by genetic methods–comparable to GFP labeling of proteins–is not …
Chemical reporters for bacterial glycans: development and applications
Bacteria possess an extraordinary repertoire of cell envelope glycans that have critical
physiological functions. Pathogenic bacteria have glycans that are essential for growth and …
physiological functions. Pathogenic bacteria have glycans that are essential for growth and …
Analytical and biochemical perspectives of protein O-GlcNAcylation
Protein O-linked β-N-acetylglucosamine (O-GlcNAc) modification (O-GlcNAcylation) is a
unique monosaccharide modification discovered in the early 1980s. With the technological …
unique monosaccharide modification discovered in the early 1980s. With the technological …
Cell membrane–camouflaged liposomes for tumor cell–selective glycans engineering and imaging in vivo
The dynamic change of cell-surface glycans is involved in diverse biological and
pathological events such as oncogenesis and metastasis. Despite tremendous efforts, it …
pathological events such as oncogenesis and metastasis. Despite tremendous efforts, it …
Advances in chemical probing of protein O-GlcNAc glycosylation: structural role and molecular mechanisms
A Saha, D Bello, A Fernández-Tejada - Chemical Society Reviews, 2021 - pubs.rsc.org
The addition of O-linked-β-DN-acetylglucosamine (O-GlcNAc) onto serine and threonine
residues of nuclear and cytoplasmic proteins is an abundant, unique post-translational …
residues of nuclear and cytoplasmic proteins is an abundant, unique post-translational …
Click-ExM enables expansion microscopy for all biomolecules
Expansion microscopy (ExM) allows super-resolution imaging on conventional fluorescence
microscopes, but has been limited to proteins and nucleic acids. Here we develop click-ExM …
microscopes, but has been limited to proteins and nucleic acids. Here we develop click-ExM …
Coupling aptamer‐based protein tagging with metabolic glycan labeling for in situ visualization and biological function study of exosomal protein‐specific …
L Zhu, Y Xu, X Wei, H Lin, M Huang, B Lin… - Angewandte …, 2021 - Wiley Online Library
Exosomal glycoproteins play important roles in many physiological and pathological
functions. Herein, we developed a dual labeling strategy based on a protein‐specific …
functions. Herein, we developed a dual labeling strategy based on a protein‐specific …
To view your biomolecule, click inside the cell
V Rigolot, C Biot, C Lion - Angewandte Chemie, 2021 - Wiley Online Library
The surging development of bioorthogonal chemistry has profoundly transformed chemical
biology over the last two decades. Involving chemical partners that specifically react together …
biology over the last two decades. Involving chemical partners that specifically react together …
Glycan labeling and analysis in cells and in vivo
B Cheng, Q Tang, C Zhang… - Annual Review of …, 2021 - annualreviews.org
As one of the major types of biomacromolecules in the cell, glycans play essential functional
roles in various biological processes. Compared with proteins and nucleic acids, the …
roles in various biological processes. Compared with proteins and nucleic acids, the …
Cell-type-specific labeling and profiling of glycans in living mice
Metabolic labeling of glycans with clickable unnatural sugars has enabled glycan analysis in
multicellular systems. However, cell-type-specific labeling of glycans in vivo remains …
multicellular systems. However, cell-type-specific labeling of glycans in vivo remains …