Protein phosphorylation is a major regulator of protein function and biological outcomes.
This was first recognized through functional biochemical experiments, and in the past …

According to Genome Sequencing Project statistics (http://www. ncbi. nlm. nih.
gov/genomes/static/gpstat. html), as of February 16, 2012, complete gene sequences have …

Lactylation was initially discovered on human histones. Given its nascence, its occurrence
on nonhistone proteins and downstream functional consequences remain elusive. Here we …

Mass spectrometry (MS) instruments and experimental protocols are rapidly advancing, but
the software tools to analyse tandem mass spectra are lagging behind. We present a …

Next-generation sequencing allows the analysis of genomes, including those representing
disease states. However, the causes of most disorders are multifactorial, and systems-level …

Degradation of cytosolic β-catenin by the APC/Axin1 destruction complex represents the key
regulated step of the Wnt pathway. It is incompletely understood how the Axin1 complex …

The adult stem cell marker Lgr5 and its relative Lgr4 are often co-expressed in Wnt-driven
proliferative compartments. We find that conditional deletion of both genes in the mouse gut …

Peptide‐centered shotgun analysis of proteins has been the core technology in mass
spectrometry based proteomics and has enabled numerous biological discoveries, such as …

Two types of stem cells are currently defined in small intestinal crypts: cycling crypt base
columnar (CBC) cells and quiescent '+ 4'cells. Here, we combine transcriptomics with …

Mass spectrometry (MS)-based phosphoproteomics has achieved extraordinary success in
qualitative and quantitative analysis of cellular protein phosphorylation. Considering that an …