Human cohesin compacts DNA by loop extrusion

Y Kim, Z Shi, H Zhang, IJ Finkelstein, H Yu - Science, 2019 - science.org
Cohesin is a chromosome-bound, multisubunit adenosine triphosphatase complex. After
loading onto chromosomes, it generates loops to regulate chromosome functions. It has …

Compartmentalization of telomeres through DNA-scaffolded phase separation

A Jack, Y Kim, AR Strom, DSW Lee, B Williams… - Developmental cell, 2022 - cell.com
Telomeres form unique nuclear compartments that prevent degradation and fusion of
chromosome ends by recruiting shelterin proteins and regulating access of DNA damage …

Large-scale preparation of fluorescence multiplex host cell reactivation (FM-HCR) reporters

CG Piett, TJ Pecen, DJ Laverty, ZD Nagel - Nature protocols, 2021 - nature.com
Repair of DNA damage is a critical survival mechanism that affects susceptibility to various
human diseases and represents a key target for cancer therapy. A major barrier to applying …

ssDNA accessibility of Rad51 is regulated by orchestrating multiple RPA dynamics

J Ding, X Li, J Shen, Y Zhao, S Zhong, L Lai… - Nature …, 2023 - nature.com
The eukaryotic single-stranded DNA (ssDNA)-binding protein Replication Protein A (RPA)
plays a crucial role in various DNA metabolic pathways, including DNA replication and …

[HTML][HTML] Condensation goes viral: a polymer physics perspective

JJ Alston, A Soranno - Journal of molecular biology, 2023 - Elsevier
The past decade has seen a revolution in our understanding of how the cellular environment
is organized, where an incredible body of work has provided new insights into the role …

How glutamate promotes liquid-liquid phase separation and DNA binding cooperativity of E. coli SSB protein

AG Kozlov, X Cheng, H Zhang, MK Shinn… - Journal of molecular …, 2022 - Elsevier
E. coli single-stranded-DNA binding protein (EcSSB) displays nearest-neighbor (NN) and
non-nearest-neighbor (NNN)) cooperativity in binding ssDNA during genome maintenance …

Polymerase theta-helicase promotes end joining by stripping single-stranded DNA-binding proteins and bridging DNA ends

JM Schaub, MM Soniat, IJ Finkelstein - Nucleic Acids Research, 2022 - academic.oup.com
Homologous recombination-deficient cancers rely on DNA polymerase Theta (Polθ)-
Mediated End Joining (TMEJ), an alternative double-strand break repair pathway. Polθ is …

[HTML][HTML] PARP1 condensates differentially partition DNA repair proteins and enhance DNA ligation

CC Sang, G Moore, M Tereshchenko, H Zhang… - EMBO …, 2024 - pmc.ncbi.nlm.nih.gov
Poly (ADP-ribose) polymerase 1 (PARP1) is one of the first responders to DNA damage and
plays crucial roles in recruiting DNA repair proteins through its activity–poly (ADP-ribosyl) …

Regulation of nearest-neighbor cooperative binding of E. coli SSB protein to DNA

AG Kozlov, MK Shinn, TM Lohman - Biophysical journal, 2019 - cell.com
Escherichia coli single-strand (ss) DNA-binding protein (SSB) is an essential protein that
binds ssDNA intermediates formed during genome maintenance. SSB homotetramers bind …

RADX condenses single-stranded DNA to antagonize RAD51 loading

H Zhang, JM Schaub, IJ Finkelstein - Nucleic acids research, 2020 - academic.oup.com
RADX is a mammalian single-stranded DNA-binding protein that stabilizes telomeres and
stalled replication forks. Cellular biology studies have shown that the balance between …