Lens-based fluorescence nanoscopy
The majority of studies of the living cell rely on capturing images using fluorescence
microscopy. Unfortunately, for centuries, diffraction of light was limiting the spatial resolution …
microscopy. Unfortunately, for centuries, diffraction of light was limiting the spatial resolution …
Measurement of nanoscale three-dimensional diffusion in the interior of living cells by STED-FCS
The observation of molecular diffusion at different spatial scales, and in particular below the
optical diffraction limit (< 200 nm), can reveal details of the subcellular topology and its …
optical diffraction limit (< 200 nm), can reveal details of the subcellular topology and its …
AIE nanoparticles with high stimulated emission depletion efficiency and photobleaching resistance for long‐term super‐resolution bioimaging
Stimulated emission depletion (STED) nanoscopy is a typical super‐resolution imaging
technique that has become a powerful tool for visualizing intracellular structures on the …
technique that has become a powerful tool for visualizing intracellular structures on the …
Encoding and decoding spatio-temporal information for super-resolution microscopy
The challenge of increasing the spatial resolution of an optical microscope beyond the
diffraction limit can be reduced to a spectroscopy task by proper manipulation of the …
diffraction limit can be reduced to a spectroscopy task by proper manipulation of the …
Background suppression in fluorescence nanoscopy with stimulated emission double depletion
P Gao, B Prunsche, L Zhou, K Nienhaus… - Nature Photonics, 2017 - nature.com
Stimulated emission depletion (STED) fluorescence nanoscopy is a powerful super-
resolution imaging technique based on the confinement of fluorescence emission to the …
resolution imaging technique based on the confinement of fluorescence emission to the …
STED nanoscopy: a glimpse into the future
The well-known saying of “Seeing is believing” became even more apt in biology when
stimulated emission depletion (STED) nanoscopy was introduced in 1994 by the Nobel …
stimulated emission depletion (STED) nanoscopy was introduced in 1994 by the Nobel …
Spatial resolution enhancement in photon-starved STED imaging using deep learning-based fluorescence lifetime analysis
As a super-resolution imaging method, stimulated emission depletion (STED) microscopy
has unraveled fine intracellular structures and provided insights into nanoscale …
has unraveled fine intracellular structures and provided insights into nanoscale …
Resolution Enhancement and Background Suppression in Optical Super‐Resolution Imaging for Biological Applications
For decades, the spatial resolution of conventional far‐field optical imaging has been
constrained due to the diffraction limit. The emergence of optical super‐resolution imaging …
constrained due to the diffraction limit. The emergence of optical super‐resolution imaging …
Low photobleaching and high emission depletion efficiency: the potential of AIE luminogen as fluorescent probe for STED microscopy
We present a preliminary study which explores the potential of aggregation-induced
emission (AIE) luminogen as a new fluorescent probe for STED microscopy. Compared with …
emission (AIE) luminogen as a new fluorescent probe for STED microscopy. Compared with …
Two-photon excitation STED microscopy with time-gated detection
We report on a novel two-photon excitation stimulated emission depletion (2PE-STED)
microscope based on time-gated detection. The time-gated detection allows for the effective …
microscope based on time-gated detection. The time-gated detection allows for the effective …