Biological nanopores: Engineering on demand

A Crnković, M Srnko, G Anderluh - Life, 2021 - mdpi.com
Nanopore-based sensing is a powerful technique for the detection of diverse organic and
inorganic molecules, long-read sequencing of nucleic acids, and single-molecule analyses …

Cytolysin A (ClyA): A Bacterial Virulence Factor with Potential Applications in Nanopore Technology, Vaccine Development, and Tumor Therapy

K Murase - Toxins, 2022 - mdpi.com
Cytolysin A (ClyA) is a pore-forming toxin that is produced by some bacteria from the
Enterobacteriaceae family. This review provides an overview of the current state of …

Enzymology on an electrode and in a nanopore: Analysis algorithms, enzyme kinetics, and perspectives

VV Shumyantseva, AV Kuzikov, RA Masamrekh… - BioNanoScience, 2022 - Springer
Enzymes are powerful and effective biological catalysts demanded in various areas of
human activity, especially in medicinal area and as modem diagnostic tools. Enzymes …

Sea anemones, actinoporins, and cholesterol

J Palacios-Ortega, D Heras-Márquez… - International Journal of …, 2022 - mdpi.com
Spanish or Spanish-speaking scientists represent a remarkably populated group within the
scientific community studying pore-forming proteins. Some of these scientists, ourselves …

Strategies for enzymological studies and measurements of biological molecules with the cytolysin A nanopore

C Wloka, NS Galenkamp, NJ van der Heide… - Methods in …, 2021 - Elsevier
Pore-forming toxins are used in a variety of biotechnological applications. Typically,
individual membrane proteins are reconstituted in artificial lipid bilayers where they form …

Hybrid lipid-block copolymer membranes enable stable reconstitution of a wide range of nanopores and robust sampling of serum

E Vreeker, F Grunewald, N van der Heide, SJ Marrink… - bioRxiv, 2024 - biorxiv.org
Biological nanopores are powerful tools for detecting biomolecules at the single-molecule
level, making them appealing as sensors for biological samples. However, the lipid …

Investigation of Heterogeneous Proteins and Protein Complexes with Native Ion Mobility-Mass Spectrometry and Theory

AD Rolland - 2022 - search.proquest.com
Native ion mobility-mass spectrometry (IM-MS) offers many advantages for the study of
biomolecules and their complexes. High mass accuracy and sensitivity enable …