As synthetic biology permeates society, the signal processing circuits in engineered living
systems must be customized to meet practical demands. Towards this mission, novel …

Highlights•Toehold-mediated strand displacement processes are increasingly utilized in
vivo.•Toehold switches enable control of gene expression in vivo and applications in …

Engineered molecular circuits that process information in biological systems could address
emerging human health and biomanufacturing needs. However, such circuits can be difficult …

Highlights•Outperforming microbial cell factories requires elaborate and programmable
devices.•Biosensors are engineered for dynamic control with various input signals.•CRISPR …

Background Biomass formation and product synthesis decoupling have been proven to be
promising to increase the titer of desired value add products. Optogenetics provides a …

Despite their successful implementation in the COVID-19 vaccines, lipid nanoparticles
(LNPs) still face a central limitation in the delivery of mRNA payloads: endosomal trapping …

Nucleic acid strand displacement reactions involve the competition of two or more DNA or
RNA strands of similar sequence for binding to a complementary strand, and facilitate the …

Multi-factor screenings are commonly used in diverse applications in medicine and
bioengineering, including optimizing combination drug treatments and microbiome …

Cotranscriptionally encoded RNA strand displacement (ctRSD) circuits are an emerging tool
for programmable molecular computation, with potential applications spanning in vitro …

Synthetic mRNA is currently produced in standardized in vitro transcription systems.
However, this one‐size‐fits‐all approach has associated drawbacks in supply chain …