CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled …

As a complex microbial aggregate, biofilm is a group behavior of bacterial ability to adapt to
the environment. Bacteria produce biofilm substrates that enhance their tolerance to stress …

Neutralizing antibodies (nAbs) elicited against the receptor binding site (RBS) of the spike
protein of wild-type severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are …

Bacteria from the same species can differ widely in their gene content. In Escherichia coli,
the set of genes shared by all strains, known as the core genome, represents about half the …

The human body is constantly exposed to microorganisms, which entails manifold
interactions between human cells and diverse commensal or pathogenic bacteria. The …

CRISPR interference (CRISPRi) is a powerful tool to link essential and nonessential genes
to specific phenotypes and to explore their functions. Here we describe a protocol for …

Functional genetic screening is an important method that has been widely used to explore
the biological processes and functional annotation of genetic elements. CRISPR/Cas …

Microbiology is at a turning point in its 120-year history. Widespread next-generation
sequencing has revealed genetic complexity among bacteria that could hardly have been …

Insects are the most diverse group of animals and colonize almost all environments on our
planet. This diversity is reflected in the structure and function of the microbial communities …

Phenotypic variation is the phenomenon in which clonal cells display different traits even
under identical environmental conditions. This plasticity is thought to be important for …