Droplet-based digital PCR (ddPCR) and its applications

Y Hou, S Chen, Y Zheng, X Zheng, JM Lin - TrAC Trends in Analytical …, 2023 - Elsevier
As a new generation absolute quantitive PCR technique, droplet-based digital PCR
(ddPCR) provides the benefits of high sensitivity, precision, and reproducibility. A low-cost …

Droplet microfluidics-based biomedical microcarriers

C Shao, J Chi, L Shang, Q Fan, F Ye - Acta biomaterialia, 2022 - Elsevier
Droplet microfluidic technology provides a new platform for controllable generation of
microdroplets and droplet-derived materials. In particular, because of the ability in high …

Recent Advances in Microfluidic Technologies for the Construction of Artificial Cells

S Tan, Y Ai, X Yin, Z Xue, X Fang… - Advanced Functional …, 2023 - Wiley Online Library
Artificial cells are synthetic constructs that emulate natural cells, with potential applications in
areas of energy science, environmental treatment, and the study of life's origins …

Rapid on-site nucleic acid testing: On-chip sample preparation, amplification, and detection, and their integration into all-in-one systems

J Wang, H Jiang, L Pan, X Gu, C Xiao, P Liu… - … in Bioengineering and …, 2023 - frontiersin.org
As nucleic acid testing is playing a vital role in increasingly many research fields, the need
for rapid on-site testing methods is also increasing. The test procedure often consists of …

Simplifying the complex: accessible microfluidic solutions for contemporary processes within in vitro diagnostics

NK Khosla, JM Lesinski, M Colombo, L Bezinge… - Lab on a Chip, 2022 - pubs.rsc.org
In vitro diagnostics (IVDs) form the cornerstone of modern medicine. They are routinely
employed throughout the entire treatment pathway, from initial diagnosis through to …

Microfluidic droplet-based functional materials for cell manipulation

Y Zheng, Z Wu, L Lin, X Zheng, Y Hou, JM Lin - Lab on a Chip, 2021 - pubs.rsc.org
Functional materials from the microfluidic-based droplet community are emerging as
enabling tools for various applications in tissue engineering and cell biology. The innovative …

Hot Flows: Evolving an Archaeal Glucose Dehydrogenase for Ultrastable Carba-NADP+ Using Microfluidics at Elevated Temperatures

I Zachos, R Genth, S Sutiono, M Marczynski… - ACS …, 2022 - ACS Publications
Here, we describe the successful engineering of a promiscuous glucose dehydrogenase
from the thermophilic archaeon, Saccharolobus solfataricus, toward the thermostable and …

Sensing area-variable electrode for wide-range droplet size detection

J Ryu, KH Han - Sensors and Actuators B: Chemical, 2024 - Elsevier
Microfluidic droplets of various sizes are applied in a broad spectrum of biological and
chemical processes and require high uniformity for reliable outcomes. Although various …

Recent advances in digital biosensing technology

K Curtin, BJ Fike, B Binkley, T Godary, P Li - Biosensors, 2022 - mdpi.com
Digital biosensing assays demonstrate remarkable advantages over conventional
biosensing systems because of their ability to achieve single-molecule detection and …

Rapid detection of hepatitis A virus in foods using a bioluminescent assay in real-time (bart) and reverse transcription loop-mediated isothermal amplification (RT …

R Wu, B Meng, M Corredig, MW Griffiths - Food and Environmental …, 2023 - Springer
Foodborne hepatitis A infections have been considered as a major threat for public health
worldwide. Increased incidences of hepatitis A virus (HAV) infection has been associated …