The ACSL4 network regulates cell death and autophagy in diseases

F Chen, R Kang, J Liu, D Tang - Biology, 2023 - mdpi.com
Simple Summary ACSL4 is an enzyme involved in the intracellular conversion of long-chain
fatty acids and coenzyme A into fatty-acid coenzymes. It plays a vital role in various …

[HTML][HTML] How to train your cell-Towards controlling phenotypes by harnessing the epigenome of Chinese hamster ovary production cell lines

N Marx, P Eisenhut, M Weinguny, G Klanert… - Biotechnology …, 2022 - Elsevier
Recent advances in omics technologies and the broad availability of big datasets have
revolutionized our understanding of Chinese hamster ovary cells in their role as the most …

Targeting group 3 medulloblastoma by the anti-PRUNE-1 and anti-LSD1/KDM1A epigenetic molecules

F Bibbò, F Asadzadeh, A Boccia, C Sorice… - International Journal of …, 2024 - mdpi.com
Medulloblastoma (MB) is a highly malignant childhood brain tumor. Group 3 MB (Gr3 MB) is
considered to have the most metastatic potential, and tailored therapies for Gr3 MB are …

Mitochondrial membrane potential-enriched CHO host: a novel and powerful tool for improving biomanufacturing capability

L Chakrabarti, R Chaerkady, J Wang, SHS Weng… - MAbs, 2022 - Taylor & Francis
With the aim of increasing protein productivity of Chinese hamster ovary (CHO) cells, we
sought to generate new CHO hosts with favorable biomanufacturing phenotypes and …

Subcloning induces changes in the DNA‐methylation pattern of outgrowing Chinese hamster ovary cell colonies

M Weinguny, G Klanert, P Eisenhut, I Lee… - Biotechnology …, 2021 - Wiley Online Library
Chinese hamster ovary (CHO) cells are the most extensively used mammalian production
system for biologics intended for use in humans. A critical step in the establishment of …

Chromosomal instability drives convergent and divergent evolution toward advantageous inherited traits in mammalian CHO bioproduction lineages

S Huhn, M Chang, A Kumar, R Liu, B Jiang… - IScience, 2022 - cell.com
Genetic instability of Chinese hamster ovary (CHO) cells is implicated in production
inconsistency through poorly defined mechanisms. Using a multi-omics approach, we …

From observational to actionable: rethinking omics in biologics production

HO Masson, KJ la Cour Karottki, J Tat, H Hefzi… - Trends in …, 2023 - cell.com
As the era of omics continues to expand with increasing ubiquity and success in both
academia and industry, omics-based experiments are becoming commonplace in industrial …

Off-gas-based soft sensor for real-time monitoring of biomass and metabolism in Chinese hamster ovary cell continuous processes in single-use bioreactors

T Wallocha, O Popp - Processes, 2021 - mdpi.com
In mammalian cell culture, especially in pharmaceutical manufacturing and research,
biomass and metabolic monitoring are mandatory for various cell culture process steps to …

[HTML][HTML] Identification of Cellular Signatures Associated with Chinese Hamster Ovary Cell Adaptation for Secretion of Antibodies.

Y Bai, ID Mercadé, R Elgendy, G Lambiase… - Computational and …, 2024 - Elsevier
The secretory capacity of Chinese hamster ovary (CHO) cells remains a fundamental
bottleneck in the manufacturing of protein-based therapeutics. Unconventional biological …

Key challenges in designing CHO chassis platforms

A Hamdi, D Széliová, DE Ruckerbauer, I Rocha… - Processes, 2020 - mdpi.com
Following the success of and the high demand for recombinant protein-based therapeutics
during the last 25 years, the pharmaceutical industry has invested significantly in the …