1Progress, applications, challenges and prospects of protein purification technology

M Du, Z Hou, L Liu, Y Xuan, X Chen, L Fan… - … in Bioengineering and …, 2022 - frontiersin.org
Protein is one of the most important biological macromolecules in life, which plays a vital
role in cell growth, development, movement, heredity, reproduction and other life activities …

Expressing cloned genes for protein production, purification, and analysis

CL Kielkopf, W Bauer… - Cold Spring Harbor …, 2021 - cshprotocols.cshlp.org
Obtaining high quantities of a specific protein directly from native sources is often
challenging, particularly when dealing with human proteins. To overcome this obstacle …

Expression of cloned genes in Pichia pastoris using the methanol-inducible promoter AOX1

CL Kielkopf, W Bauer… - Cold Spring Harbor …, 2021 - cshprotocols.cshlp.org
Pichia pastoris is a methylotrophic yeast capable of metabolizing methanol as its sole
carbon source. Growth in methanol-containing medium results in dramatic induction of …

Intracellular production of recombinant GnRH1 in yeast, Pichia pastoris, and its potential as oral treatment to advance gonadal development in juvenile orange-spotted …

J Nocillado, P Palma, T Wang, EG de Jesus-Ayson… - Aquaculture, 2022 - Elsevier
Late maturation and large size at maturity are significant challenges when administering
hormonal treatments in captive broodstock. Here, we report the potential of yeast, Pichia …