Past, present and future of ICSI in livestock species

O Briski, DF Salamone - Animal Reproduction Science, 2022 - Elsevier
During the past 2 decades, intracytoplasmic sperm injection (ICSI) has become a routine
technique for clinical applications in humans. The widespread use among domestic species …

Intracytoplasmic sperm injection in domestic and wild mammals

DF Salamone, NG Canel, MB Rodríguez - Reproduction, 2017 - rep.bioscientifica.com
Intracytoplasmic sperm injection (ICSI) has become a useful technique for clinical
applications in the horse-breeding industry. However, both ICSI blastocyst and offspring …

Sperm selection by thermotaxis improves ICSI outcome in mice

S Pérez-Cerezales, R Laguna-Barraza… - Scientific Reports, 2018 - nature.com
The ejaculate is a heterogeneous pool of spermatozoa containing only a small
physiologically adequate subpopulation for fertilization. As there is no method to isolate this …

Transient sperm starvation improves the outcome of assisted reproductive technologies

FA Navarrete, L Aguila, D Martin-Hidalgo… - Frontiers in cell and …, 2019 - frontiersin.org
To become fertile, mammalian sperm must undergo a series of biochemical and
physiological changes known as capacitation. These changes involve crosstalk between …

Bovine ICSI: Limiting factors, strategies to improve its efficiency and alternative approaches

F Fuentes, E Munoz, MJ Contreras, ME Arias, R Felmer - Zygote, 2022 - cambridge.org
Intracytoplasmic sperm injection (ICSI) is an assisted reproductive technique mainly used to
overcome severe infertility problems associated with the male factor, but in cattle its …

Intracytoplasmic sperm injection in cattle

V Unnikrishnan, J Kastelic, J Thundathil - Genes, 2021 - mdpi.com
Intracytoplasmic sperm injection (ICSI) involves the microinjection of sperm into a matured
oocyte. Although this reproductive technology is successfully used in humans and many …

DMSO supplementation during in vitro maturation of bovine oocytes improves blastocyst rate and quality

AE Ynsaurralde-Rivolta, M Suvá, CG Luchetti… - Theriogenology, 2020 - Elsevier
The molecule Dimethyl sulfoxide is widely used as drug solvent. However, its antioxidant
property was poorly explored. In this study, we evaluated the effect of DMSO …

Improved embryo development using high cysteamine concentration during IVM and sperm co-culture with COCs previous to ICSI in bovine

NG Canel, M Suvá, RJ Bevacqua, ME Arias, R Felmer… - Theriogenology, 2018 - Elsevier
In contrast to other species, intracytoplasmic sperm injection (ICSI) in bovine remains
inefficient, resulting in low embryo developmental rates. It is unclear whether such …

Production of bovine embryos by piezo-ICSI using capacitated spermatozoa selected by fluorescence-activated cell sorting (FACS-piezo-ICSI)

M Castro, L Aguila, ME Arias, R Felmer - Animal Reproduction Science, 2024 - Elsevier
Intracytoplasmic sperm injection (ICSI) remains inefficient in cattle. One reason could lie in
the injection of oocytes with sperm that have not undergone molecular changes associated …

Human sperm decondensation in vitro is related to cleavage rate and embryo quality in IVF

C Galotto, MY Cambiasso, VL Julianelli… - Journal of Assisted …, 2019 - Springer
Purpose To investigate whether the ability of human spermatozoa to decondense in vitro in
the presence of heparin (Hep) and glutathione (GSH) is related to assisted reproduction …