Past, present and future of ICSI in livestock species
O Briski, DF Salamone - Animal Reproduction Science, 2022 - Elsevier
During the past 2 decades, intracytoplasmic sperm injection (ICSI) has become a routine
technique for clinical applications in humans. The widespread use among domestic species …
technique for clinical applications in humans. The widespread use among domestic species …
Intracytoplasmic sperm injection in domestic and wild mammals
DF Salamone, NG Canel, MB Rodríguez - Reproduction, 2017 - rep.bioscientifica.com
Intracytoplasmic sperm injection (ICSI) has become a useful technique for clinical
applications in the horse-breeding industry. However, both ICSI blastocyst and offspring …
applications in the horse-breeding industry. However, both ICSI blastocyst and offspring …
Sperm selection by thermotaxis improves ICSI outcome in mice
S Pérez-Cerezales, R Laguna-Barraza… - Scientific Reports, 2018 - nature.com
The ejaculate is a heterogeneous pool of spermatozoa containing only a small
physiologically adequate subpopulation for fertilization. As there is no method to isolate this …
physiologically adequate subpopulation for fertilization. As there is no method to isolate this …
Transient sperm starvation improves the outcome of assisted reproductive technologies
To become fertile, mammalian sperm must undergo a series of biochemical and
physiological changes known as capacitation. These changes involve crosstalk between …
physiological changes known as capacitation. These changes involve crosstalk between …
Bovine ICSI: Limiting factors, strategies to improve its efficiency and alternative approaches
Intracytoplasmic sperm injection (ICSI) is an assisted reproductive technique mainly used to
overcome severe infertility problems associated with the male factor, but in cattle its …
overcome severe infertility problems associated with the male factor, but in cattle its …
Intracytoplasmic sperm injection in cattle
V Unnikrishnan, J Kastelic, J Thundathil - Genes, 2021 - mdpi.com
Intracytoplasmic sperm injection (ICSI) involves the microinjection of sperm into a matured
oocyte. Although this reproductive technology is successfully used in humans and many …
oocyte. Although this reproductive technology is successfully used in humans and many …
DMSO supplementation during in vitro maturation of bovine oocytes improves blastocyst rate and quality
AE Ynsaurralde-Rivolta, M Suvá, CG Luchetti… - Theriogenology, 2020 - Elsevier
The molecule Dimethyl sulfoxide is widely used as drug solvent. However, its antioxidant
property was poorly explored. In this study, we evaluated the effect of DMSO …
property was poorly explored. In this study, we evaluated the effect of DMSO …
Improved embryo development using high cysteamine concentration during IVM and sperm co-culture with COCs previous to ICSI in bovine
NG Canel, M Suvá, RJ Bevacqua, ME Arias, R Felmer… - Theriogenology, 2018 - Elsevier
In contrast to other species, intracytoplasmic sperm injection (ICSI) in bovine remains
inefficient, resulting in low embryo developmental rates. It is unclear whether such …
inefficient, resulting in low embryo developmental rates. It is unclear whether such …
Production of bovine embryos by piezo-ICSI using capacitated spermatozoa selected by fluorescence-activated cell sorting (FACS-piezo-ICSI)
Intracytoplasmic sperm injection (ICSI) remains inefficient in cattle. One reason could lie in
the injection of oocytes with sperm that have not undergone molecular changes associated …
the injection of oocytes with sperm that have not undergone molecular changes associated …
Human sperm decondensation in vitro is related to cleavage rate and embryo quality in IVF
C Galotto, MY Cambiasso, VL Julianelli… - Journal of Assisted …, 2019 - Springer
Purpose To investigate whether the ability of human spermatozoa to decondense in vitro in
the presence of heparin (Hep) and glutathione (GSH) is related to assisted reproduction …
the presence of heparin (Hep) and glutathione (GSH) is related to assisted reproduction …