Conserved and divergent mechanisms of inner kinetochore assembly onto centromeric chromatin

S Yatskevich, D Barford, KW Muir - Current Opinion in Structural Biology, 2023 - Elsevier
Kinetochores are large protein complexes built on centromeric chromatin that mediate
chromosome segregation. The inner kinetochore, or constitutive centromere-associated …

[HTML][HTML] Histone variants and chromatin structure, update of advances

V Sokolova, S Sarkar, D Tan - Computational and Structural Biotechnology …, 2023 - Elsevier
Histone proteins are highly conserved among all eukaryotes. They have two important
functions in the cell: to package the genomic DNA and to regulate gene accessibility …

Cryo-EM structure of the complete inner kinetochore of the budding yeast point centromere

T Dendooven, Z Zhang, J Yang, SH McLaughlin… - Science …, 2023 - science.org
The point centromere of budding yeast specifies assembly of the large kinetochore complex
to mediate chromatid segregation. Kinetochores comprise the centromere-associated inner …

Phase-plate cryo-EM structure of the Widom 601 CENP-A nucleosome core particle reveals differential flexibility of the DNA ends

R Boopathi, R Danev, M Khoshouei, S Kale… - Nucleic acids …, 2020 - academic.oup.com
The histone H3 variant CENP-A marks centromeres epigenetically and is essential for
mitotic fidelity. Previous crystallographic studies of the CENP-A nucleosome core particle …

[HTML][HTML] Recent advances in single-molecule fluorescence microscopy render structural biology dynamic

L Bacic, A Sabantsev, S Deindl - Current opinion in structural biology, 2020 - Elsevier
Highlights•Single-molecule fluorescence microscopy is a powerful tool to study the structural
dynamics of biological macromolecules.•Recent developments in multi-coordinate single …

Are extraordinary nucleosome structures more ordinary than we thought?

CYY Chong, L Gan - Chromosoma, 2023 - Springer
The nucleosome is a DNA–protein assembly that is the basic unit of chromatin. A
nucleosome can adopt various structures. In the canonical nucleosome structure, 145–147 …

Structural insights into p300 regulation and acetylation-dependent genome organisation

Z Ibrahim, T Wang, O Destaing, N Salvi… - Nature …, 2022 - nature.com
Histone modifications are deposited by chromatin modifying enzymes and read out by
proteins that recognize the modified state. BRD4-NUT is an oncogenic fusion protein of the …

Structural and dynamic mechanisms of CBF3-guided centromeric nucleosome formation

R Guan, T Lian, BR Zhou, E He, C Wu… - Nature …, 2021 - nature.com
Accurate chromosome segregation relies on the specific centromeric nucleosome–
kinetochore interface. In budding yeast, the centromere CBF3 complex guides the …

CENP-A nucleosome—a chromatin-embedded pedestal for the centromere: lessons learned from structural biology

A Ali-Ahmad, N Sekulić - Essays in Biochemistry, 2020 - portlandpress.com
The centromere is a chromosome locus that directs equal segregation of chromosomes
during cell division. A nucleosome containing the histone H3 variant CENP-A epigenetically …

Methylation of CENP-A/Cse4 on arginine 143 and lysine 131 regulates kinetochore stability in yeast

TM Tran Nguyen, A Munhoven… - Genetics, 2023 - academic.oup.com
Post-translational modifications on histones are well known to regulate chromatin structure
and function, but much less information is available on modifications of the centromeric …