Cells confront DNA damage in every cell cycle. Among the most deleterious types of DNA
damage are DNA double-strand breaks (DSBs), which can cause cell lethality if unrepaired …

Human papillomavirus (HPV) is the most common sexually transmitted virus in the world.
About 70% of cervical cancers are caused by the most oncogenic HPV genotypes of 16 and …

The response to DNA damage is critical for cellular homeostasis, tumor suppression,
immunity, and gametogenesis. In order to provide an unbiased and global view of the DNA …

CRISPR/Cas9 gene editing can inactivate genes in a precise manner. This process involves
DNA double-strand breaks (DSB), which may incur a loss of cell fitness. We hypothesize that …

The ability to precisely modify human genes has been made possible by the development of
tools such as meganucleases, zinc finger nucleases, TALENs, and CRISPR/Cas. These now …

Recent studies have reported that genome editing by CRISPR–Cas9 induces a DNA
damage response mediated by p53 in primary cells hampering their growth. This could lead …

Canonical CRISPR–knockout (KO) screens rely on Cas9-induced DNA double-strand
breaks (DSBs) to generate targeted gene KOs. These methodologies may yield distorted …

Inactivating p53 mutations are the most abundant genetic alterations found in cancer. Here
we show that CRISPR/Cas9-induced double-stranded DNA breaks enrich for cells deficient …

Cancer 'genetic dependencies'—genes whose products are essential for cancer cell fitness—
are promising targets for therapeutic development. However, recent evidence has cast doubt …

Functional genomics describes a field of biology that uses a range of approaches for
assessing gene function with high-throughput molecular, genetic, and cellular technologies …