The exploration of protease substrate specificity is generally restricted to naturally occurring
amino acids, limiting the degree of conformational space that can be surveyed. We …

In this review we describe in detail the available technologies used for investigating the
substrate specificity of proteases. Critical comparison of the available detection methods and …

Protease‐catalyzed hydrolysis of peptide bonds is one of the most pivotal post‐translational
modifications fulfilling manifold functions in the regulation of cellular processes. Therefore …

Matrix metalloproteinases (MMPs) and A Disintegrin and Metalloproteinases (ADAMs) are
two related protease families that play key roles in matrix remodeling and growth factor …

Fragment-based ligand design (FBLD) approaches have become more widely used in drug
discovery projects from both academia and industry, and are even often preferred to …

Semliki Forest virus (SFV) is a member of the Alphavirus genus, which produces its
replicase proteins in the form of a nonstructural (ns) polyprotein precursor P1234. The …

The ability to follow enzyme activity in a cellular context represents a challenging
technological frontier that impacts fields ranging from disease pathogenesis to epigenetics …

Substrate specificity of proteases can be determined using several methods among which
the most frequently used are positional scanning library, proteomics and phage display …

Knowledge of cleavage site specificity and activity are major prerequisites for understanding
protease function. On the basis of a recently presented approach for proteomic identification …

Proteases are involved in almost all biological processes, and therefore, aberrant activity of
many of these enzymes is an important indicator of disease. Various methods have been …