Towards organoid culture without Matrigel

MT Kozlowski, CJ Crook, HT Ku - Communications biology, 2021 - nature.com
Organoids—cellular aggregates derived from stem or progenitor cells that recapitulate organ
function in miniature—are of growing interest in developmental biology and medicine …

Recent trends in protein and peptide-based biomaterials for advanced drug delivery

A Varanko, S Saha, A Chilkoti - Advanced drug delivery reviews, 2020 - Elsevier
Engineering protein and peptide-based materials for drug delivery applications has gained
momentum due to their biochemical and biophysical properties over synthetic materials …

Genomic RNA elements drive phase separation of the SARS-CoV-2 nucleocapsid

C Iserman, CA Roden, MA Boerneke, RSG Sealfon… - Molecular cell, 2020 - cell.com
We report that the SARS-CoV-2 nucleocapsid protein (N-protein) undergoes liquid-liquid
phase separation (LLPS) with viral RNA. N-protein condenses with specific RNA genomic …

Elastin-like polypeptides for biomedical applications

AK Varanko, JC Su, A Chilkoti - Annual review of biomedical …, 2020 - annualreviews.org
Elastin-like polypeptides (ELPs) are stimulus-responsive biopolymers derived from human
elastin. Their unique properties—including lower critical solution temperature phase …

Stimuli-responsive, pentapeptide, nanofiber hydrogel for tissue engineering

JD Tang, C Mura, KJ Lampe - Journal of the American Chemical …, 2019 - ACS Publications
Short peptides are uniquely versatile building blocks for self-assembly. Supramolecular
peptide assemblies can be used to construct functional hydrogel biomaterials—an attractive …

Sequence heuristics to encode phase behaviour in intrinsically disordered protein polymers

FG Quiroz, A Chilkoti - Nature materials, 2015 - nature.com
Proteins and synthetic polymers that undergo aqueous phase transitions mediate self-
assembly in nature and in man-made material systems. Yet little is known about how the …

Coacervate droplets for synthetic cells

Z Lin, T Beneyton, JC Baret, N Martin - Small Methods, 2023 - Wiley Online Library
The design and construction of synthetic cells–human‐made microcompartments that mimic
features of living cells–have experienced a real boom in the past decade. While many efforts …

[HTML][HTML] Elastin-like polypeptides as models of intrinsically disordered proteins

S Roberts, M Dzuricky, A Chilkoti - FEBS letters, 2015 - Elsevier
Elastin-like polypeptides (ELPs) are a class of stimuli-responsive biopolymers inspired by
the intrinsically disordered domains of tropoelastin that are composed of repeats of the …

Advances in understanding stimulus-responsive phase behavior of intrinsically disordered protein polymers

KM Ruff, S Roberts, A Chilkoti, RV Pappu - Journal of molecular biology, 2018 - Elsevier
Proteins and synthetic polymers can undergo phase transitions in response to changes to
intensive solution parameters such as temperature, proton chemical potentials (pH), and …

Cononsolvency of thermoresponsive polymers: where we are now and where we are going

S Bharadwaj, BJ Niebuur, K Nothdurft, W Richtering… - Soft Matter, 2022 - pubs.rsc.org
Cononsolvency is an intriguing phenomenon where a polymer collapses in a mixture of
good solvents. This cosolvent-induced modulation of the polymer solubility has been …