Microfluidic systems for single DNA dynamics

DJ Mai, C Brockman, CM Schroeder - Soft matter, 2012 - pubs.rsc.org
Recent advances in microfluidics have enabled the molecular-level study of polymer
dynamics using single DNA chains. Single polymer studies based on fluorescence …

Recent advances in single‐molecule detection on micro‐and nano‐fluidic devices

C Liu, Y Qu, Y Luo, N Fang - Electrophoresis, 2011 - Wiley Online Library
Single‐molecule detection (SMD) allows static and dynamic heterogeneities from seemingly
equal molecules to be revealed in the studies of molecular structures and intra‐and inter …

Multiplexed miRNA quantitation using injectionless microfluidic thermal gel electrophoresis

MA Cornejo, TH Linz - Analytical chemistry, 2022 - ACS Publications
MicroRNAs (miRNAs) are a class of biomolecules that have high clinical and
pharmaceutical significance because of their ability to regulate protein expression. Better …

Micronozzle array enhanced sandwich electroporation of embryonic stem cells

Z Fei, X Hu, H Choi, S Wang, D Farson… - Analytical …, 2010 - ACS Publications
Electroporation is one of the most popular nonviral gene transfer methods for embryonic
stem cell transfection. Bulk electroporation techniques, however, require a high electrical …

[HTML][HTML] Microfluidic converging/diverging channels optimised for homogeneous extensional deformation

K Zografos, F Pimenta, MA Alves, MSN Oliveira - Biomicrofluidics, 2016 - pubs.aip.org
In this work, we optimise microfluidic converging/diverging geometries in order to produce
constant strain-rates along the centreline of the flow, for performing studies under …

The use of microfluidics in rheology

X Hu, PE Boukany, OL Hemminger… - Macromolecular …, 2011 - Wiley Online Library
The combination of microfluidics and fluorescent‐labeled DNA molecules in rheology
provides a unique opportunity to relate the microstructure of polymer molecules to their …

Optimization of flow-focusing devices for homogeneous extensional flow

F Pimenta, RG Sousa, MA Alves - Biomicrofluidics, 2018 - pubs.aip.org
We present a methodology for the shape optimization of flow-focusing devices with the
purpose of creating a wide region of homogeneous extensional flow, characterized by a …

Effect of the electrostatic interactions on stretching of semiflexible and biological polyelectrolytes

JMY Carrillo, AV Dobrynin - Macromolecules, 2010 - ACS Publications
Using combination of the molecular dynamics simulations and theoretical calculations, we
have demonstrated that the bending rigidity of biological polyelectrolytes (semiflexible …

Simulation of conformational preconditioning strategies for electrophoretic stretching of DNA in a microcontraction

CC Hsieh, TH Lin - Biomicrofluidics, 2011 - pubs.aip.org
We have used Brownian dynamics-finite element method to examine two conformational
preconditioning approaches for improving DNA stretching in a microcontraction for the …

Development of Microfluidic Thermal Gel Electrophoresis for Biological Analysis

MA Cornejo - 2024 - search.proquest.com
The molecular biology that takes place within cells is vital to the overall function of an
organism. Healthy cellular function is crucial to a wide web of interconnected processes that …