Recombinant protein expression in Escherichia coli: advances and challenges
GL Rosano, EA Ceccarelli - Frontiers in microbiology, 2014 - frontiersin.org
Escherichia coli is one of the organisms of choice for the production of recombinant proteins.
Its use as a cell factory is well-established and it has become the most popular expression …
Its use as a cell factory is well-established and it has become the most popular expression …
Strategies for the Production of Recombinant Protein in Escherichia coli
In the recent past years, a large number of proteins have been expressed in Escherichia coli
with high productivity due to rapid development of genetic engineering technologies. There …
with high productivity due to rapid development of genetic engineering technologies. There …
Microbial synthesis of vanillin from waste poly (ethylene terephthalate)
JC Sadler, S Wallace - Green Chemistry, 2021 - pubs.rsc.org
Poly (ethylene terephthalate)(PET) is an abundant and extremely useful material, with
widespread applications across society. However, there is an urgent need to develop …
widespread applications across society. However, there is an urgent need to develop …
Synonymous codons direct cotranslational folding toward different protein conformations
F Buhr, S Jha, M Thommen, J Mittelstaet, F Kutz… - Molecular cell, 2016 - cell.com
In all genomes, most amino acids are encoded by more than one codon. Synonymous
codons can modulate protein production and folding, but the mechanism connecting codon …
codons can modulate protein production and folding, but the mechanism connecting codon …
Tunable thermal bioswitches for in vivo control of microbial therapeutics
Temperature is a unique input signal that could be used by engineered microbial
therapeutics to sense and respond to host conditions or spatially targeted external triggers …
therapeutics to sense and respond to host conditions or spatially targeted external triggers …
Biomolecular ultrasound and sonogenetics
Visualizing and modulating molecular and cellular processes occurring deep within living
organisms is fundamental to our study of basic biology and disease. Currently, the most …
organisms is fundamental to our study of basic biology and disease. Currently, the most …
[HTML][HTML] The growing world of small heat shock proteins: from structure to functions
S Carra, S Alberti, PA Arrigo, JL Benesch… - Cell Stress and …, 2017 - Elsevier
Small heat shock proteins (sHSPs) are present in all kingdoms of life and play fundamental
roles in cell biology. sHSPs are key components of the cellular protein quality control …
roles in cell biology. sHSPs are key components of the cellular protein quality control …
The heat shock response in moss plants is regulated by specific calcium-permeable channels in the plasma membrane
Land plants are prone to strong thermal variations and must therefore sense early moderate
temperature increments to induce appropriate cellular defenses, such as molecular …
temperature increments to induce appropriate cellular defenses, such as molecular …
Current strategies for protein production and purification enabling membrane protein structural biology
Membrane proteins are still heavily under-represented in the protein data bank (PDB),
owing to multiple bottlenecks. The typical low abundance of membrane proteins in their …
owing to multiple bottlenecks. The typical low abundance of membrane proteins in their …
[HTML][HTML] Production of prone-to-aggregate proteins
M Lebendiker, T Danieli - FEBS letters, 2014 - Elsevier
Expression of recombinant proteins in Escherichia coli (E. coli) remains the most popular
and cost-effective method for producing proteins in basic research and for pharmaceutical …
and cost-effective method for producing proteins in basic research and for pharmaceutical …