Cytochrome c oxidase (CcO), the terminal oxidase of cell respiration, reduces molecular
oxygen (O2) in a reaction that is coupled with proton pumping. Since the discovery of this …

Cytochrome c oxidase (CcO) is a terminal protein of the respiratory chain in eukaryotes and
some bacteria. It catalyzes most of the biologic oxygen consumption on earth done by …

The crystal structures of cytochrome c oxidase from both bovine and Paracoccus
denitrificans reveal two putative proton input channels that connect the heme-copper center …

Elucidating the structures of intermediates in the reduction of O2 to water by cytochrome c
oxidase is crucial to understanding both oxygen activation and proton pumping by the …

Revised Manuscript Received December 29, 1993• abstract: We have studiedthe flow-flash
reaction of fully reduced cytochrome c oxidase with a high concentration of oxygen (1 mM) …

Cytochrome c oxidase is a membrane-bound enzyme that catalyzes the four-electron
reduction of oxygen to water. This highly exergonic reaction drives proton pumping across …

The current status of our knowledge about the mechanism of proton pumping by cytochrome
oxidase is discussed. Significant progress has resulted from the study of site-directed …

MATERIALS AND METHODS Dodecyl/3-D-maltoside (DM) and 30% hydrogen peroxide
were purchased from Aldrich. Tris (hydroxymethyl) ami-nomethane (Tris), V-(2-acetoamido) …

The “peroxy” intermediate (P form) of bovine cytochrome c oxidase was prepared by
reaction of the two-electron reduced mixed-valence CO complex with 18O2 after photolytic …

Cytochrome bd is one of the two terminal quinol oxidases in the respiratory chain of
Escherichia coli. The enzyme catalyzes charge separation across the bacterial membrane …