Engineered enzymes for the synthesis of pharmaceuticals and other high-value products
MT Reetz, G Qu, Z Sun - Nature Synthesis, 2024 - nature.com
Catalysis has a central role in organic synthetic methodology, especially in stereoselective
reactions. In many reactions, enantioselectivity is made possible through the use of chiral …
reactions. In many reactions, enantioselectivity is made possible through the use of chiral …
Running the numbers on plant synthetic biology solutions to global problems
Synthetic biology and metabolic engineering promise to deliver sustainable solutions to
global problems such as phasing out fossil fuels and replacing industrial nitrogen fixation …
global problems such as phasing out fossil fuels and replacing industrial nitrogen fixation …
A pilot oral history of plant synthetic biology
The whole field of synthetic biology (SynBio) is only about 20 years old, and plant SynBio is
younger still. Nevertheless, within that short time, SynBio in general has drawn more …
younger still. Nevertheless, within that short time, SynBio in general has drawn more …
Synthetic Biology─ High time to deliver?
Synthetic biology (SynBio) has attracted like no other recent development the attention not
only of Life Science researchers and engineers but also of intellectuals, technology think …
only of Life Science researchers and engineers but also of intellectuals, technology think …
Automated in vivo enzyme engineering accelerates biocatalyst optimization
E Orsi, L Schada von Borzyskowski, S Noack… - Nature …, 2024 - nature.com
Achieving cost-competitive bio-based processes requires development of stable and
selective biocatalysts. Their realization through in vitro enzyme characterization and …
selective biocatalysts. Their realization through in vitro enzyme characterization and …
Synthetically-primed adaptation of Pseudomonas putida to a non-native substrate D-xylose
To broaden the substrate scope of microbial cell factories towards renewable substrates,
rational genetic interventions are often combined with adaptive laboratory evolution (ALE) …
rational genetic interventions are often combined with adaptive laboratory evolution (ALE) …
CRISPR-COPIES: an in silico platform for discovery of neutral integration sites for CRISPR/Cas-facilitated gene integration
AG Boob, Z Zhu, P Intasian, M Jain… - Nucleic acids …, 2024 - academic.oup.com
The CRISPR/Cas system has emerged as a powerful tool for genome editing in metabolic
engineering and human gene therapy. However, locating the optimal site on the …
engineering and human gene therapy. However, locating the optimal site on the …
Ionic-liquid-tolerant enzymes from an adapted Bacillus paralicheniformis for one-pot conversion of lignocellulosic substrates to ethanol
A newly isolated thermophilic cellulase-producing Bacillus paralicheniformis was subjected
to adaptive laboratory evolution in the presence of increasing concentration (from 1 to 5 …
to adaptive laboratory evolution in the presence of increasing concentration (from 1 to 5 …
Advances in the discovery and engineering of gene targets for carotenoid biosynthesis in recombinant strains
B Su, MR Deng, H Zhu - Biomolecules, 2023 - mdpi.com
Carotenoids are naturally occurring pigments that are abundant in the natural world. Due to
their excellent antioxidant attributes, carotenoids are widely utilized in various industries …
their excellent antioxidant attributes, carotenoids are widely utilized in various industries …
Enabling pathway design by multiplex experimentation and machine learning
AG Boob, J Chen, H Zhao - Metabolic Engineering, 2024 - Elsevier
The remarkable metabolic diversity observed in nature has provided a foundation for
sustainable production of a wide array of valuable molecules. However, transferring the …
sustainable production of a wide array of valuable molecules. However, transferring the …