[HTML][HTML] Evolving SAXS versatility: solution X-ray scattering for macromolecular architecture, functional landscapes, and integrative structural biology

CA Brosey, JA Tainer - Current opinion in structural biology, 2019 - Elsevier
Highlights•SAXS captures architecture and dynamics to elucidate molecular
ensembles.•SAXS advances now probe key thermodynamic and kinetic properties of …

Methods for physical characterization of phase-separated bodies and membrane-less organelles

DM Mitrea, B Chandra, MC Ferrolino, EB Gibbs… - Journal of molecular …, 2018 - Elsevier
Membrane-less organelles are cellular structures which arise through the phenomenon of
phase separation. This process enables compartmentalization of specific sets of …

[PDF][PDF] BioXTAS RAW: improvements to a free open-source program for small-angle X-ray scattering data reduction and analysis

JB Hopkins, RE Gillilan, S Skou - Journal of applied crystallography, 2017 - journals.iucr.org
BioXTAS RAW is a graphical-user-interface-based free open-source Python program for
reduction and analysis of small-angle X-ray solution scattering (SAXS) data. The software is …

Domain movements upon activation of phenylalanine hydroxylase characterized by crystallography and chromatography-coupled small-angle X-ray scattering

SP Meisburger, AB Taylor, CA Khan… - Journal of the …, 2016 - ACS Publications
Mammalian phenylalanine hydroxylase (PheH) is an allosteric enzyme that catalyzes the
first step in the catabolism of the amino acid phenylalanine. Following allosteric activation by …

X-ray scattering studies of protein structural dynamics

SP Meisburger, WC Thomas, MB Watkins… - Chemical …, 2017 - ACS Publications
X-ray scattering is uniquely suited to the study of disordered systems and thus has the
potential to provide insight into dynamic processes where diffraction methods fail. In …

[HTML][HTML] Recent advances in small-angle scattering and its expanding impact in structural biology

J Trewhella - Structure, 2022 - cell.com
Applications of small-angle scattering (SAS) in structural biology have benefited from
continuing developments in instrumentation, tools for data analysis, modeling capabilities …

[PDF][PDF] US-SOMO HPLC-SAXS module: dealing with capillary fouling and extraction of pure component patterns from poorly resolved SEC-SAXS data

E Brookes, P Vachette, M Rocco… - Journal of applied …, 2016 - journals.iucr.org
Size-exclusion chromatography coupled with SAXS (small-angle X-ray scattering), often
performed using a flow-through capillary, should allow direct collection of monodisperse …

An optimized SEC-SAXS system enabling high X-ray dose for rapid SAXS assessment with correlated UV measurements for biomolecular structure analysis

TM Ryan, J Trewhella, JM Murphy… - Journal of applied …, 2018 - journals.iucr.org
A new optimized size exclusion chromatography small-angle X-ray scattering (SEC-SAXS)
system for biomolecular SAXS at the Australian Synchrotron SAXS/WAXS beamline has …

Advances on size exclusion chromatography and applications on the analysis of protein biopharmaceuticals and protein aggregates: a mini review

G Brusotti, E Calleri, R Colombo, G Massolini… - Chromatographia, 2018 - Springer
Biopharmaceutical drugs are large, complex protein molecules derived from living cells. The
characterization of the protein-based products is a complicated and challenging task …

[PDF][PDF] Progress in small-angle scattering from biological solutions at high-brilliance synchrotrons

AT Tuukkanen, A Spilotros, DI Svergun - IUCrJ, 2017 - journals.iucr.org
Small-angle X-ray scattering (SAXS) is an established technique that provides low-
resolution structural information on macromolecular solutions. Recent decades have …