Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …

Fluorescence lifetime imaging (FLIM) uses the fact that the fluorescence lifetime of a
fluorophore depends on its molecular environment but not on its concentration. Molecular …

The function of a T cell depends on its subtype and activation state. Here, we show that
imaging of the autofluorescence lifetime signals of quiescent and activated T cells can be …

There is a growing interest in developing microphysiological systems that can be used to
model both normal and pathological human organs in vitro. This “organs-on-chips” …

NAD is a key determinant of cellular energy metabolism. In contrast, its phosphorylated form,
NADP, plays a central role in biosynthetic pathways and antioxidant defence. The reduced …

Fluorescence lifetime imaging microscopy (FLIM) is used in diverse disciplines, including
biology, chemistry and biophysics, but its use has been limited by the complexity of the data …

Much of the mechanism by which Wnt signaling drives proliferation during oncogenesis is
attributed to its regulation of the cell cycle. Here, we show how Wnt/β‐catenin signaling …

Skin is a widely used route of delivery for local and systemic drugs and is potentially a route
for their delivery as nanoparticles. The skin provides a natural physical barrier against …

Mitochondria display complex morphology and movements, which complicates their
segmentation and tracking in time-lapse images. Here, we introduce Mitometer, an algorithm …

Molecular, morphological, and physiological heterogeneity is the inherent property of cells
which governs differences in their response to external influence. Tumor cell metabolic …