Chromosome Duplication in Saccharomyces cerevisiae
SP Bell, K Labib - Genetics, 2016 - academic.oup.com
The accurate and complete replication of genomic DNA is essential for all life. In eukaryotic
cells, the assembly of the multi-enzyme replisomes that perform replication is divided into …
cells, the assembly of the multi-enzyme replisomes that perform replication is divided into …
Mechanisms and regulation of DNA replication initiation in eukaryotes
MW Parker, MR Botchan, JM Berger - Critical reviews in …, 2017 - Taylor & Francis
Cellular DNA replication is initiated through the action of multiprotein complexes that
recognize replication start sites in the chromosome (termed origins) and facilitate duplex …
recognize replication start sites in the chromosome (termed origins) and facilitate duplex …
The mechanism of eukaryotic CMG helicase activation
The initiation of eukaryotic DNA replication occurs in two discrete stages: first, the
minichromosome maintenance (MCM) complex assembles as a head-to-head double …
minichromosome maintenance (MCM) complex assembles as a head-to-head double …
Structure of the eukaryotic MCM complex at 3.8 Å
DNA replication in eukaryotes is strictly regulated by several mechanisms. A central step in
this replication is the assembly of the heterohexameric minichromosome maintenance …
this replication is the assembly of the heterohexameric minichromosome maintenance …
[HTML][HTML] Single-molecule studies of origin licensing reveal mechanisms ensuring bidirectional helicase loading
Loading of the ring-shaped Mcm2–7 replicative helicase around DNA licenses eukaryotic
origins of replication. During loading, Cdc6, Cdt1, and the origin-recognition complex (ORC) …
origins of replication. During loading, Cdc6, Cdt1, and the origin-recognition complex (ORC) …
Structure of eukaryotic CMG helicase at a replication fork and implications to replisome architecture and origin initiation
The eukaryotic CMG (Cdc45, Mcm2–7, GINS) helicase consists of the Mcm2–7 hexameric
ring along with five accessory factors. The Mcm2–7 heterohexamer, like other hexameric …
ring along with five accessory factors. The Mcm2–7 heterohexamer, like other hexameric …
MCM: one ring to rule them all
TD Deegan, JFX Diffley - Current opinion in structural biology, 2016 - Elsevier
Highlights•The eukaryotic MCM replicative helicase is tightly regulated during the cell
cycle.•Conformational changes in MCM underpin the different stages of chromosome …
cycle.•Conformational changes in MCM underpin the different stages of chromosome …
The architecture of a eukaryotic replisome
At the eukaryotic DNA replication fork, it is widely believed that the Cdc45–Mcm2–7–GINS
(CMG) helicase is positioned in front to unwind DNA and that DNA polymerases trail behind …
(CMG) helicase is positioned in front to unwind DNA and that DNA polymerases trail behind …
Structure of the eukaryotic replicative CMG helicase suggests a pumpjack motion for translocation
The CMG helicase is composed of Cdc45, Mcm2–7 and GINS. Here we report the structure
of the Saccharomyces cerevisiae CMG, determined by cryo-EM at a resolution of 3.7–4.8 Å …
of the Saccharomyces cerevisiae CMG, determined by cryo-EM at a resolution of 3.7–4.8 Å …
Mcm10 regulates DNA replication elongation by stimulating the CMG replicative helicase
M Lõoke, MF Maloney, SP Bell - Genes & development, 2017 - genesdev.cshlp.org
Activation of the Mcm2–7 replicative DNA helicase is the committed step in eukaryotic DNA
replication initiation. Although Mcm2–7 activation requires binding of the helicase-activating …
replication initiation. Although Mcm2–7 activation requires binding of the helicase-activating …