Overview of optical and electrochemical alkaline phosphatase (ALP) biosensors: Recent approaches in cells culture techniques

T Balbaied, E Moore - Biosensors, 2019 - mdpi.com
Alkaline phosphatase (ALP), which catalyzes the dephosphorylation process of proteins,
nucleic acids, and small molecules, can be found in a variety of tissues (intestine, liver …

Chemical analysis of single cells

R Trouillon, MK Passarelli, J Wang, ME Kurczy… - Analytical …, 2013 - ACS Publications
Many biological systems contain an ensemble of cells with heterogeneous chemistry;
therefore, it is important to analyze them on an individual basis in order to elucidate the role …

Intracellular electrochemical sensing

K Ino, Y Nashimoto, N Taira, JR Azcon… - Electroanalysis, 2018 - Wiley Online Library
Observing biochemical processes within living cell is imperative for biological and medical
research. Fluoresce imaging is widely used for intracellular sensing of cell membranes …

Improving the electrochemical imaging sensitivity of scanning electrochemical microscopy-scanning ion conductance microscopy by using electrochemical Pt …

M Şen, Y Takahashi, Y Matsumae… - Analytical …, 2015 - ACS Publications
We fabricated a platinum-based double barrel probe for scanning electrochemical
microscopy–scanning ion conductance microscopy (SECM–SICM) by electrodepositing …

Nanoporous carbon-fiber microelectrodes for sensitive detection of H2O2 and dopamine

F Seven, T Gölcez, ŞEN Mustafa - Journal of Electroanalytical Chemistry, 2020 - Elsevier
In this study, sensitive electrochemical detection of H 2 O 2 and dopamine (DA) was
demonstrated using a nanoporous carbon fiber electrode (CFE). SEM images confirmed the …

Novel electrochemical methodology for activity estimation of alkaline phosphatase based on solubility difference

K Ino, Y Kanno, T Arai, KY Inoue, Y Takahashi… - Analytical …, 2012 - ACS Publications
We propose a novel electrochemical detection system for alkaline phosphatase (ALP)
activity using the difference in water and oil solubilities between the substrate, ferrocene …

LSI-based amperometric sensor for real-time monitoring of embryoid bodies

M Şen, K Ino, KY Inoue, T Arai, T Nishijo, A Suda… - Biosensors and …, 2013 - Elsevier
A large scale integration (LSI)-based amperometric sensor is used for electrochemical
evaluation and real-time monitoring of the alkaline phosphatase (ALP) activity of mouse …

Accumulation and detection of secreted proteins from single cells for reporter gene assays using a local redox cycling-based electrochemical (LRC-EC) chip device

M Şen, K Ino, H Shiku, T Matsue - Lab on a Chip, 2012 - pubs.rsc.org
A lab-on-a-chip device is described for the electrochemical detection of alkaline
phosphatase (ALP) secreted by transformed single HeLa cells. Detection on the chip device …

Multiplex cell microarrays for high-throughput screening

OI Berthuy, SK Muldur, F Rossi, P Colpo, LJ Blum… - Lab on a Chip, 2016 - pubs.rsc.org
Microarray technology was developed in the early 1990s to measure the transcription levels
of thousands of genes in parallel. The basic premise of high-density arraying has since been …

Sensitive PH measurement using EGFET PH-microsensor based on ZnO nanowire functionalized carbon-fibers

A Aykaç, ID Tunç, F Güneş, M Erol, M Şen - Nanotechnology, 2021 - iopscience.iop.org
Herein, we report the fabrication of zinc oxide nanowire (ZnO NW) coated carbon fiber (CF)
ultra-microelectrodes (UME). ZnO NWs were grown on commercial multifilament CFs …