One of the major objectives of the aquaculture industry is the production of a large number of
viable eggs with high survival. Major achievements have been made in recent years in …

Morpholino oligonucleotides are the most common anti-sense “knockdown” technique used
in zebrafish (Danio rerio). This review discusses common practices for the design …

CRISPR-Cas9 technology provides a powerful system for genome engineering. However,
variable activity across different single guide RNAs (sgRNAs) remains a significant …

The zebrafish (Danio rerio) has emerged as a powerful model to study vertebrate
development and disease. Its short generation time makes it amenable to genetic …

Early embryonic development is driven exclusively by maternal gene products deposited
into the oocyte. Although critical in establishing early developmental programs, maternal …

MicroRNAs (miRNAs) are small RNAs that regulate gene expression posttranscriptionally.
To block all miRNA formation in zebrafish, we generated maternal-zygotic dicer (MZ dicer) …

The signals directing primordial germ cell (PGC) migration in vertebrates are largely
unknown. We demonstrate that sdf-1 mRNA is expressed in locations where PGCs are …

Recent studies have revealed unexpected connections between the mammalian Hedgehog
(Hh) signal transduction pathway and the primary cilium, a microtubule-based organelle that …

The combination of genome editing and primordial germ cell (PGC) transplantation has
enormous significance in the study of developmental biology and genetic breeding, despite …

In most animals, primordial germ cell (PGC) specification and development depend on
maternally provided cytoplasmic determinants that constitute the so-called germ plasm [1–5] …