Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments

M Gantz, S Neun, EJ Medcalf, LD van Vliet… - Chemical …, 2023 - ACS Publications
Novel and improved biocatalysts are increasingly sourced from libraries via experimental
screening. The success of such campaigns is crucially dependent on the number of …

Recent advances in droplet microfluidics

Y Ding, PD Howes, AJ deMello - Analytical chemistry, 2019 - ACS Publications
Prominent within this technology set are droplet-based microfluidic systems, 3 in which
passive microfluidic structures are used to rapidly generate and manipulate subnanoliter …

Materials and methods for droplet microfluidic device fabrication

KS Elvira, F Gielen, SSH Tsai, AM Nightingale - Lab on a Chip, 2022 - pubs.rsc.org
Since the first reports two decades ago, droplet-based systems have emerged as a
compelling tool for microbiological and (bio) chemical science, with droplet flow providing …

Speeding up enzyme discovery and engineering with ultrahigh-throughput methods

HA Bunzel, X Garrabou, M Pott, D Hilvert - Current opinion in structural …, 2018 - Elsevier
Exploring the sequence space of enzyme catalysts is ultimately a numbers game. Ultrahigh-
throughput screening methods for rapid analysis of millions of variants are therefore …

Recent advances in the design of microfluidic technologies for the manufacture of drug releasing particles

A Forigua, RL Kirsch, SM Willerth, KS Elvira - Journal of Controlled Release, 2021 - Elsevier
Drug releasing particles are valued for their ability to deliver therapeutics to targeted
locations and for their controllable release patterns. The development of microfluidic …

Simple and precise counting of viable bacteria by resazurin-amplified picoarray detection

K Hsieh, HC Zec, L Chen, AM Kaushik… - Analytical …, 2018 - ACS Publications
Simple, fast, and precise counting of viable bacteria is fundamental to a variety of
microbiological applications such as food quality monitoring and clinical diagnosis. To this …

Microfluidic technologies for drug discovery and development: friend or foe?

KS Elvira - Trends in Pharmacological Sciences, 2021 - cell.com
There is a point in the evolution of every new technology when questions need to be asked
regarding its usefulness and impact. Although microfluidic technologies have drastically …

A programmable and automated optical electrowetting-on-dielectric (oEWOD) driven platform for massively parallel and sequential processing of single cell assay …

LG Welch, J Estranero, P Tourlomousis, RCR Wootton… - Lab on a Chip, 2024 - pubs.rsc.org
Recently, there has been an increasing emphasis on single cell profiling for high-throughput
screening workflows in drug discovery and life sciences research. However, the biology …

From single-molecule detection to next-generation sequencing: microfluidic droplets for high-throughput nucleic acid analysis

Y Ding, J Choo, AJ DeMello - Microfluidics and nanofluidics, 2017 - Springer
Droplet-based microfluidic technologies have proved themselves to be of significant utility in
the performance of high-throughput chemical and biological experiments. By encapsulating …

Microfluidic technique for the simultaneous quantification of emulsion instabilities and lipid digestion kinetics

N Scheuble, A Iles, RCR Wootton, EJ Windhab… - Analytical …, 2017 - ACS Publications
Quantifying the impact of environmental physicochemical changes on the microstructure of
lipid delivery systems is challenging. Therefore, we have developed a methodology to …