Recent advances in crop transformation technologies

Z Chen, JM Debernardi, J Dubcovsky, A Gallavotti - Nature plants, 2022 - nature.com
Agriculture is experiencing a technological inflection point in its history, while also facing
unprecedented challenges posed by human population growth and global climate changes …

[HTML][HTML] CRISPR/Cas-mediated plant genome editing: outstanding challenges a decade after implementation

T Cardi, J Murovec, A Bakhsh, J Boniecka… - Trends in Plant …, 2023 - cell.com
The discovery of the CRISPR/Cas genome-editing system has revolutionized our
understanding of the plant genome. CRISPR/Cas has been used for over a decade to …

[HTML][HTML] Heritable transgene-free genome editing in plants by grafting of wild-type shoots to transgenic donor rootstocks

L Yang, F Machin, S Wang, E Saplaoura… - Nature …, 2023 - nature.com
Generation of stable gene-edited plant lines using clustered regularly interspaced short
palindromic repeats (CRISPR)–CRISPR-associated protein 9 (Cas9) requires a lengthy …

[HTML][HTML] Cut–dip–budding delivery system enables genetic modifications in plants without tissue culture

X Cao, H Xie, M Song, J Lu, P Ma, B Huang, M Wang… - The Innovation, 2023 - cell.com
Of the more than 370 000 species of higher plants in nature, fewer than 0.1% can be
genetically modified due to limitations of the current gene delivery systems. Even for those …

[HTML][HTML] Engineered biocontainable RNA virus vectors for non-transgenic genome editing across crop species and genotypes

Q Liu, C Zhao, K Sun, Y Deng, Z Li - Molecular Plant, 2023 - cell.com
CRISPR/Cas genome-editing tools provide unprecedented opportunities for basic plant
biology research and crop breeding. However, the lack of robust delivery methods has …

[HTML][HTML] Challenges facing CRISPR/Cas9-based genome editing in plants

S Son, SR Park - Frontiers in plant science, 2022 - frontiersin.org
The development of plant varieties with desired traits is imperative to ensure future food
security. The revolution of genome editing technologies based on the clustered regularly …

Subcellular compartmentalization in the biosynthesis and engineering of plant natural produces

L Yao, X Wu, X Jiang, M Shan, Z Zhang, Y Li… - Biotechnology …, 2023 - Elsevier
Plant natural products (PNPs) are specialized metabolites with diverse bioactivities. They
are extensively used in the pharmaceutical, cosmeceutical and food industries. PNPs are …

Evolution and origin of bread wheat

AA Levy, M Feldman - The Plant Cell, 2022 - academic.oup.com
Bread wheat (Triticum aestivum, genome BBAADD) is a young hexaploid species formed
only 8,500–9,000 years ago through hybridization between a domesticated free-threshing …

[HTML][HTML] Virus-induced gene silencing (VIGS): a powerful tool for crop improvement and its advancement towards epigenetics

S Zulfiqar, MA Farooq, T Zhao, PP Wang… - International journal of …, 2023 - mdpi.com
Virus-induced gene silencing (VIGS) is an RNA-mediated reverse genetics technology that
has evolved into an indispensable approach for analyzing the function of genes. It …

Fighting wheat powdery mildew: from genes to fields

B Wang, T Meng, B Xiao, T Yu, T Yue, Y Jin… - Theoretical and Applied …, 2023 - Springer
Key message Host resistance conferred by Pm genes provides an effective strategy to
control powdery mildew. The study of Pm genes helps modern breeding develop toward …