Environmental DNA metabarcoding: Transforming how we survey animal and plant communities

K Deiner, HM Bik, E Mächler, M Seymour… - Molecular …, 2017 - Wiley Online Library
The genomic revolution has fundamentally changed how we survey biodiversity on earth.
High‐throughput sequencing (“HTS”) platforms now enable the rapid sequencing of DNA …

Fishing for fish environmental DNA: Ecological applications, methodological considerations, surveying designs, and ways forward

M Yao, S Zhang, Q Lu, X Chen, SY Zhang… - Molecular …, 2022 - Wiley Online Library
Vast global declines of freshwater and marine fish diversity and population abundance pose
serious threats to both ecosystem sustainability and human livelihoods. Environmental DNA …

Critical considerations for the application of environmental DNA methods to detect aquatic species

CS Goldberg, CR Turner, K Deiner… - Methods in ecology …, 2016 - Wiley Online Library
Species detection using environmental DNA (eDNA) has tremendous potential for
contributing to the understanding of the ecology and conservation of aquatic species …

The ecology of environmental DNA and implications for conservation genetics

MA Barnes, CR Turner - Conservation genetics, 2016 - Springer
Environmental DNA (eDNA) refers to the genetic material that can be extracted from bulk
environmental samples such as soil, water, and even air. The rapidly expanding study of …

MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: detection of more than 230 subtropical marine species

M Miya, Y Sato, T Fukunaga, T Sado… - Royal Society …, 2015 - royalsocietypublishing.org
We developed a set of universal PCR primers (MiFish-U/E) for metabarcoding
environmental DNA (eDNA) from fishes. Primers were designed using aligned whole …

[HTML][HTML] Environmental DNA–An emerging tool in conservation for monitoring past and present biodiversity

PF Thomsen, E Willerslev - Biological conservation, 2015 - Elsevier
The continuous decline in Earth's biodiversity represents a major crisis and challenge for the
21st century, and there is international political agreement to slow down or halt this decline …

Methodology of fish eDNA and its applications in ecology and environment

S Wang, Z Yan, B Hänfling, X Zheng, P Wang… - Science of the Total …, 2021 - Elsevier
Fish environmental DNA (eDNA) studies have made substantial progress during the past
decade, and significant advances in monitoring fishes have been gained by taking …

Environmental DNA metabarcoding reveals local fish communities in a species-rich coastal sea

S Yamamoto, R Masuda, Y Sato, T Sado, H Araki… - Scientific reports, 2017 - nature.com
Environmental DNA (eDNA) metabarcoding has emerged as a potentially powerful tool to
assess aquatic community structures. However, the method has hitherto lacked field tests …

The detection of aquatic macroorganisms using environmental DNA analysis—A review of methods for collection, extraction, and detection

S Tsuji, T Takahara, H Doi, N Shibata… - Environmental …, 2019 - Wiley Online Library
The field of environmental DNA (eDNA) analysis has rapidly developed over the past
decade and the technique has become widely used for detecting aquatic macroorganisms in …

Environmental DNA for wildlife biology and biodiversity monitoring

K Bohmann, A Evans, MTP Gilbert, GR Carvalho… - Trends in ecology & …, 2014 - cell.com
Extraction and identification of DNA from an environmental sample has proven noteworthy
recently in detecting and monitoring not only common species, but also those that are …