RNA therapeutics (eg siRNA, oligonucleotides, mRNA, etc.) show great potential for the
treatment of a myriad of diseases. However, to reach their site of action in the cytosol or …

Eukaryotic cells employ diverse uptake mechanisms depending on their specialized
functions. While such mechanisms vary widely in their defining criteria: scale, molecular …

Intracellular organelles change their size during trafficking and maturation. This requires the
transport of ions and water across their membranes. Macropinocytosis, a ubiquitous form of …

The phosphatase FIG4 and the scaffold protein VAC14 function in the biosynthesis of PI (3,
5) P2, a signaling lipid that inhibits the lysosomal chloride transporter ClC-7. Loss-of …

Abstract The mannose-6-phosphate (M6P) biosynthetic pathway for lysosome biogenesis
has been studied for decades and is considered a well-understood topic. However, whether …

The interpretation of high throughput sequencing data is limited by our incomplete functional
understanding of coding and non-coding transcripts. Reliably predicting the function of such …

Background In recent years, large-scale genetic screens using the CRISPR/Cas9 system
have emerged as scalable approaches able to interrogate gene function with …

Enzymes that produce second messengers are highly regulated. Revealing the mechanisms
underlying such regulation is critical to understanding both how cells achieve specific …

The acidic luminal pH of lysosomes, maintained within a narrow range, is essential for
proper degrative function of the organelle and is generated by the action of a V-type H+ …

Background Volume-regulated anion channels (VRACs) are heterohexamers of LRRC8A
with LRRC8B,-C,-D, or-E in various combinations. Depending on the subunit composition …