A comprehensive summary of a decade development of the recombinase polymerase amplification
J Li, J Macdonald, F Von Stetten - Analyst, 2019 - pubs.rsc.org
Nucleic acid amplification has permeated every field in the life sciences since the
introduction of the classic polymerase chain reaction (PCR) method in 1983. Yet, despite its …
introduction of the classic polymerase chain reaction (PCR) method in 1983. Yet, despite its …
Recombinase polymerase amplification: Basics, applications and recent advances
IM Lobato, CK O'Sullivan - Trac Trends in analytical chemistry, 2018 - Elsevier
Recombinase polymerase amplification (RPA) is a highly sensitive and selective isothermal
amplification technique, operating at 37–42° C, with minimal sample preparation and …
amplification technique, operating at 37–42° C, with minimal sample preparation and …
Recombinase polymerase amplification for diagnostic applications
RK Daher, G Stewart, M Boissinot… - Clinical …, 2016 - academic.oup.com
BACKGROUND First introduced in 2006, recombinase polymerase amplification (RPA) has
stirred great interest, as evidenced by 75 publications as of October 2015, with 56 of them …
stirred great interest, as evidenced by 75 publications as of October 2015, with 56 of them …
Rapid and fully microfluidic Ebola virus detection with CRISPR-Cas13a
Highly infectious illness caused by pathogens is endemic especially in developing nations
where there is limited laboratory infrastructure and trained personnel. Rapid point-of-care …
where there is limited laboratory infrastructure and trained personnel. Rapid point-of-care …
Advances in isothermal amplification: novel strategies inspired by biological processes
J Li, J Macdonald - Biosensors and Bioelectronics, 2015 - Elsevier
Nucleic acid amplification is an essential process in biological systems. The in vitro adoption
of this process has resulted in powerful techniques that underpin modern molecular biology …
of this process has resulted in powerful techniques that underpin modern molecular biology …
Suitcase lab for rapid detection of SARS-CoV-2 based on recombinase polymerase amplification assay
AA El Wahed, P Patel, M Maier, C Pietsch… - Analytical …, 2021 - ACS Publications
In March 2020, the SARS-CoV-2 virus outbreak was declared as a world pandemic by the
World Health Organization (WHO). The only measures for controlling the outbreak are …
World Health Organization (WHO). The only measures for controlling the outbreak are …
Critical insight into recombinase polymerase amplification technology
MA Munawar - Expert Review of Molecular Diagnostics, 2022 - Taylor & Francis
Introduction Recombinase polymerase amplification (RPA) is a promising and emerging
technology for rapidly amplifying target nucleic acid from minimally processed samples and …
technology for rapidly amplifying target nucleic acid from minimally processed samples and …
Recombinase polymerase amplification: Emergence as a critical molecular technology for rapid, low-resource diagnostics
A James, J Macdonald - Expert review of molecular diagnostics, 2015 - Taylor & Francis
Isothermal molecular diagnostics are bridging the technology gap between traditional
diagnostics and polymerase chain reaction-based methods. These new techniques enable …
diagnostics and polymerase chain reaction-based methods. These new techniques enable …
Influence of sequence mismatches on the specificity of recombinase polymerase amplification technology
RK Daher, G Stewart, M Boissinot, DK Boudreau… - Molecular and cellular …, 2015 - Elsevier
Recombinase polymerase amplification (RPA) technology relies on three major proteins,
recombinase proteins, single-strand binding proteins, and polymerases, to specifically …
recombinase proteins, single-strand binding proteins, and polymerases, to specifically …
Droplet digital recombinase polymerase amplification (ddRPA) reaction unlocking via picoinjection
Recombinase polymerase amplification (RPA) has been recognized as a promising
isothermal amplification method for nucleic acid detection. However, the digital format of …
isothermal amplification method for nucleic acid detection. However, the digital format of …