Our ability to engineer protein structure and function has grown dramatically over recent
years. Perhaps the next level in protein design is to develop proteins whose function can be …

Deep mutational scanning has been used to create high-resolution DNA sequence maps
that illustrate the functional consequences of large numbers of point mutations. However …

A fundamental goal of protein biochemistry is to determine the sequence–function
relationship, but the vastness of sequence space makes comprehensive evaluation of this …

Circular permutation (CP) involves the cleavage of polypeptide to obtain new termini, which
can result in different protein structures and functions. It has been demonstrated to be an …

Protein insertional fusion and circular permutation are 2 promising protein engineering
techniques for creating integrated functionalities and sequence diversity of a protein …

Circularly permuted proteins (cpPs) represent a novel type of mutant proteins with original
termini that are covalently linked through a peptide connector and opened at any other place …